UPLC同时测定清热止咳颗粒中7种黄酮类成分含量  

Simultaneous determination of seven flavonoids in Qingre Zhike Granules by UPLC

作　　者：李鸷 余淑美 余代鑫 于娟 袁慧君 林少华 徐伟[1] LI Zhi;YU Shumei;YU Daixin;YU Juan;YUAN Huijun;LIN Shaohua;XU Wei(Pharmacy college of Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Zhangzhou Pien Tze Huang Parmaceutical Co.,Ltd.,Zhangzhou 363000,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]漳州片仔癀药业股份有限公司,福建漳州363000

出　　处：《药学研究》2020年第5期272-277,共6页Journal of Pharmaceutical Research

基　　金：中央引导地方科技发展专项(No.2018L3016);福建省经典中药复方创新与再评价研究中心(No.2017L3017)。

摘　　要：目的建立同时测定清热止咳颗粒中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、黄芩苷、木蝴蝶素A-7-葡萄糖醛酸苷、汉黄芩苷含量的方法。方法采用超高效液相色谱法(UPLC),采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),流动相乙腈-0.1%甲酸水溶液(梯度洗脱),流速0.3 m L·min^-1,检测波长280 nm,柱温30℃。结果芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、黄芩苷、木蝴蝶素A-7-葡萄糖醛酸苷、汉黄芩苷分别在0.324~6.480(r=0.999 9),1.337~26.75(r=0.999 7),0.687~13.80(r=0.999 6),1.347~26.95(r=0.999 8),2.770~55.40(r=0.999 2),0.330~6.600(r=0.999 8),0.242~4.840(r=0.999 6)μg·m L-1范围内与峰面积呈良好的线性关系;并且芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、黄芩苷、木蝴蝶素A-7-葡萄糖醛酸苷、汉黄芩苷平均加样回收率分别为99.41%、101.80%、99.42%、98.47%、101.71%、102.27%和97.64%,RSD范围分别是2.32%、1.69%、2.38%、2.45%、1.24%、1.77%和1.91%。结论建立的超高效液相色谱方法操作简便、稳定、准确且可行,可用于清热止咳颗粒的质量控制。Objective To establish an UPLC method for the simultaneous determination of narirutin,naringin,hesperidin,neohesperidin,baicalin,oroxylin A-7-glucoronide and wogonoside in Qingre Zhike Granules. Methods UPLC was performed on ACQUITY UPLC BEH C18 chromatographic column( 2.1 mm×100 mm,1.7 μm) with mobile phase acetonitrile-0.1% formic acid( gradient elution) at a flow rate of 0.3 m L·min^-1.The detction wavelength was set at 280 nm,and the column temperature was 30 ℃ .Results Narirutin,naringin,hesperidin,neohesperidin,baicalin,oroxylin A-7-glucoronide and wogonoside showed good linear relationship between concentration and peak area within the ranges of 0.324 ~ 6.480( r =0.999 9),1.337 ~ 26.75( r = 0.999 7),0.687 ~ 13.80( r = 0.999 6),1.347 ~ 26.95( r = 0.999 8),2.770 ~ 55.40( r = 0.999 2),0.330 ~ 6.600( r = 0. 999 8),0. 242 ~ 4. 840( r = 0. 999 6) μg · m L-1,respectively. The average recoveries were 99.41%,101.80%,99. 42%,98. 47%,101. 71%,102. 27% and 97. 64%,respectively for narirutin,naringin,hesperidin,neohesperidin,baicalin,oroxylin A-7-glucoronide and wogonoside,with RSDs of 2.32%,1.69%,2.38%,2. 45%,1. 24%,1.77% and 1.91%.Conclusion The results indicated that the new developed method could be simple,stable,accurate and feasible for the quality control of Qingre Zhike Granules.

关 键 词：清热止咳颗粒 超高效液相色谱法 芸香柚皮苷 柚皮苷 橙皮苷 新橙皮苷 黄芩苷 木蝴蝶素A-7-葡萄糖醛酸苷 汉黄芩苷 

分 类 号：R927.2[医药卫生—药学]