不同年龄女性卵泡液及颗粒细胞的氧化应激状态对胚胎质量的影响  

Effects of Oxidative Stress in Follicular Environment on Embryo Quality in Women of Different Ages

作　　者：温灿鑫 李瑞岐[1] 区颂邦[1] 杨冬梓[1] 李予[1] WEN Can-xin;LI Rui-qi;OU Song-bang;YANG Dong-zi;LI Yu(Reproductive Center,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China)

机构地区：[1]中山大学孙逸仙纪念医院生殖中心,广东广州510120

出　　处：《中山大学学报（医学科学版）》2020年第3期465-472,共8页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广州市科技计划项目产学研协同创新重大专项民生科技专题(201704020046);中华医学会临床科研专项基金(18010170746)。

摘　　要：【目的】探讨不同生育年龄段女性取卵周期中卵泡液及颗粒细胞的氧化应激状态及其与胚胎质量的关系。【方法】收集2018年9月8日至2019年1月18日在本中心行新鲜体外受精胚胎移植助孕的不孕患者取卵日卵泡液共70例,按照年龄≥35岁及<35岁分为年长组(n=34)和年轻组(n=36),比较卵泡液中氧化应激3个阶段的7项指标及颗粒细胞抗氧化物的基因表达水平,分析两组氧化应激指标的差异及其与胚胎质量的关系。【结果】年长组卵泡液的过氧化氢(H2O2)水平显著增加[155.84(137.11~199.30)vs.66.94(51.05~95.42)μmol/L,P=0.001],总超氧化物歧化酶(SOD)活性显著降低[0.69(0.55~0.85)vs.0.83(0.76~0.94)U,P=0.005],年长组颗粒细胞的过氧化氢酶(CAT)和谷胱甘肽还原酶(GSR)表达量显著下降(P<0.05)。卵泡液总SOD活性在正常受精率≥60%组、囊胚形成率≥60%组均较高[0.82(0.71~0.91)vs.0.67(0.55~0.80)U,P=0.007,0.83(0.78~0.90)vs.0.73(0.60~0.89)U,P=0.049],并且经相关因素校正后卵泡液总SOD活性高对正常受精率(≥60%)及囊胚形成率(≥60%)的影响有统计学意义(OR值分别为5.478、3.383,95%CI分别为1.721~17.433、1.111~10.299,P值分别为0.004、0.032)。【结论】卵泡液和颗粒细胞的氧化应激状态在高龄女性中主要表现为卵泡液ROS增加、总SOD活性降低,颗粒细胞中过氧化氢酶及谷胱甘肽还原酶的水平显著降低,并且卵泡液总SOD活性与正常受精率和囊胚形成率呈正相关,提示其对卵子质量可能存在一定的影响。【Objective】To investigate the oxidative stress status in follicular fluid and granulosa cells obtained during oocyte retrieval cycles among women of different ages and to explore the relationship between the oxidative stress and the embryo quality.【Methods】From September 8,2018 to January 18,2019 in our reproductive center,70 follicular fluid samples on the day of oocyte retrieval were collected from infertile women undergoing in vitro fertilization(IVF)or intra?cytoplasmic sperm injection(ICSI).We divided the samples into those at or above(aged group,n=34)and below the age of 35(young group,n=36)and compared seven oxidative stress markers in follicular fluid and gene expression of antioxidant enzymes in granulosa cells,and then analyzed their differences between groups and their relationship with embryo development parameters.【Results】We observed significantly higher concentration of hydrogen peroxide(H2O2)[155.84(137.11~199.30)vs.66.94(51.05~95.42)μmol/L,P=0.001],significantly lower total superoxide dismutase(SOD)activity[0.69(0.55~0.85)vs.0.83(0.76~0.94)U,P=0.005],decreased catalase(CAT)and glutathione reduc?tase(GSR)expression(P<0.05)in women with advanced materal age.Total SOD activity in follicular fluid was statisti?cally higher both in patients with normal fertilization rate(≥60%)and with blastocyst formation rate(≥60%)[0.82(0.71~0.91)vs.0.67(0.55~0.80)U,P=0.007,0.83(0.78~0.90)vs.0.73(0.60~0.89)U,P=0.049].Multivariate logistic analysis revealed that higher total SOD activity correlated with normal fertilization rate(≥60%)and blastocyst forma?tion rate(≥60%),with 5.478 and 3.383 for odds ratio(OR)values,1.721~17.433 and 1.111~10.299 for 95%CI,0.004 and 0.032 for P values,respectively.【Conclusions】The oxidative stress in follicular environment among women with advanced materal age is mainly characterized by increased level of reactive oxygen species(ROS),decreased SOD activity in follicular fluid and reduced expression of CAT and GSR in granulosa cells.Moreover,total SOD activity in

关 键 词：卵泡液 超氧化物歧化酶 卵巢老化 囊胚形成率 颗粒细胞 

分 类 号：R711.6[医药卫生—妇产科学]