新生儿缺氧缺血性脑病MMP-2基因多态性及其与炎性因子表达的关系研究  

Study on the polymorphism of matrix MMP-2 gene in neonatal hypoxic-ischemic encephalopathy and its relationship with inflammatory factor expression

作　　者：兰雪[1] 路明 王竹颖[1] Lan Xue;Lu Ming;Wang Zhuying(Department of Neonatal Pediatrics,the First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院新生儿科,哈尔滨150001

出　　处：《中国医学前沿杂志（电子版）》2020年第5期122-125,共4页Chinese Journal of the Frontiers of Medical Science(Electronic Version)

摘　　要：目的探讨新生儿缺氧缺血性脑病(hypoxic ischemic encephalopathy,HIE)MMP-2基因多态性及其与炎性因子表达的关系。方法将2016年1月至2018年12月哈尔滨医科大学附属第一医院收治的60例HIE新生儿纳入观察组,另将同期于该院分娩的60例健康新生儿纳入对照组。比较两组新生儿MMP-2多态性位点基因型和炎性因子水平,以及观察组MMP-2多态性位点不同基因型新生儿血清炎性因子水平。结果观察组中CC基因型新生儿占比显著高于对照组(P=0.020),CT基因型新生儿占比显著低于对照组(P=0.049),两组TT基因型新生儿占比比较差异无统计学意义(P>0.05)。观察组新生儿血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素-6(interleukin-6,IL-6)水平均显著高于对照组(均P<0.05),且观察组中CC基因型新生儿血清TNF-α、IL-6水平均显著高于CT基因型和TT基因型新生儿(均P<0.05)。结论HIE新生儿MMP-2基因多态性以CC基因型为主,且与HIE患儿体内血清TNF-α、IL-6表达有关,CC基因型可能通过加重炎性反应参与HIE的发病过程。Objective To explore the MMP-2 gene in neonatal hypoxic ischemic encephalopathy(HIE)and its relationship with inflammatory factor expression.Method 60 HIE newborns admitted to the First Affiliated Hospital of Harbin Medical University from January 2016 to December 2018 were included in observation group,and 60 healthy newborns delivered in the same period were included in control group.To compare the genotypes and inflammatory factors of MMP-2 polymorphism site in the two groups,and the levels of serum inflammatory factors of the newborns with different genotypes in MMP-2 polymorphism site.Result The proportion of newborns with CC genotype in observation group was significantly higher than that in control group(P=0.020),the proportion of newborns with CT genotype was significantly lower than that in control group(P=0.049),and there was no significant difference in the proportion of TT genotype between the two groups(P>0.05).The levels of serum tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in observation group were significantly higher than those in control group(all P<0.05),and the levels of serum TNF-α and IL-6 of newborns with CC genotype in observation group were significantly higher than those with CT genotype and TT genotype(all P<0.05).Conclusion The MMP-2 gene polymorphism of HIE newborn is mainly based on the CC genotype and is related to the expression of serum TNF-αand IL-6 in HIE newborn.The CC genotype may participate in the pathogenesis of HIE by aggravating the inflammatory response.

关 键 词：基质金属蛋白酶-2 基因多态性 新生儿 缺氧缺血性脑病 炎性因子 

分 类 号：R722.1[医药卫生—儿科]