盐度对硝酸细菌活性的影响及动力学特性  

Effect of salinity on activity of nitrite-oxidizing bacteria and kinetic characterization

作　　者：张宇坤[1,2,3] 张亮 董怡君[1] 顾升波[1] 彭永臻[1] ZHANG Yu-kun;ZHANG Liang;DONG Yi-jun;GU Sheng-bo;PENG Yong-zhen(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Beijing University of Technology,Beijing 100124,China;School of Civil Engineering,Dalian Nationalities University,Dalian 116600,China;Jiangsu Tianyu Environmental protection Group CO.Ltd.,Yangzhou 225200,China)

机构地区：[1]北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室,北京100124 [2]大连民族大学,土木工程学院,辽宁大连116600 [3]江苏天雨环保集团有限公司,江苏扬州225200

出　　处：《中国环境科学》2020年第5期2047-2052,共6页China Environmental Science

基　　金：国家自然科学基金资助项目(51978007);中央高校基本科研业务费专项资金资助项目(wd01129);大学生创新创业训练计划项目(201912026426,201912026419)。

摘　　要：为分析盐度对硝酸细菌(NOB)活性的影响及其动力学特性,本文采用高浓度亚硝态氮污水富集培养NOB.对NOB富集污泥进行荧光原位杂交技术(FISH)分析表明Nitrobacter占细菌总数的(81%±6%).污泥的最大比亚硝态氮氧化速率为(42.5±0.9)mgN/(gVSS·h).用此污泥考察了盐度对NOB活性的影响,并测定了盐度为10g/L时NOB的动力学参数(Ko、KS).结果表明,与盐度为0g/L时的NOB活性相比,盐度为15g/L时NOB活性降低了3.3%;盐度为10和20g/L时的NOB活性分别降低了11%.盐度为10g/L时,NOB的最大比亚硝态氮氧化速率为(37.9±0.7)mgN/(gVSS·h),氧的半饱和常数Ko值为(1.51±0.06)mg/L,底物(亚硝态氮)半饱和常数KS值为(6.06±0.15)mg/L,Ko、KS测定值均高于ASm^2模型中氨氧化细菌(AOB)推荐值.盐度对NOB的抑制降低了最大比亚硝态氮氧化速率,对氧传递和底物(亚硝态氮)传递均存在影响.In order to analyze the effect of salinity on NOB(nitrite-oxidizing bacteria)activity and kinetic characterization,NOB was enriched in a lab-scale SBR system with high concentration of nitrite wastewater in this study.The FISH(fluorescence in situ hybridization)analysis showed that Nitrobacter accounted for(81%±6%)of total bacteria.The maximum specific nitrite oxidation rate was(42.5±0.9)mgN/(gVSS·h).The effect of salinity on NOB activity was investigated using this mixed culture.The kinetic parameters of NOB(Ko,KS)were both measured under salinity of 10g/L.Results showed that compared with salinity 0g/L,the activity of NOB decreased 3.3%unders alinity of 15g/L and decreased 11%under salinity of 10,20 g/L.When salinity was 10g/L,the maximum specific nitrite oxidation rate was(37.9±0.7)mgN/(gVSS·h).The half-saturation constant for oxygen(Ko)and nitrite(KS)were(1.51±0.06),(6.06±0.15)mg/L.The values of Ko and KS were higher than recommended value of ammonia-oxidizing bacteria(AOB)in ASm^2 model.It is suggested that salinity could reduce maximum specific nitrite oxidation rate and affect the oxygen affinity and substrate(nitrite)affinity of NOB.

关 键 词：活性 污水 动力学 硝酸细菌 盐度 

分 类 号：X703.1[环境科学与工程—环境工程]