不同温度下BDE-209对草鱼离体肝脏组织的毒性效应  

Toxic effects of BDE-209 on isolated liver tissues of Ctenopharyngodon idellus at different temperatures

作　　者：解华晓 钟利桥[2] 吴路银 姚凡 倪朝辉[2] XIE Hua-xiao;ZHONG Li-qiao;WU Lu-yin;YAO Fan;NI Zhao-hui(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Fishery Eco-environmental Monitoring Center in the Upper and Middle Reaches of the Yangtze River,Ministry of Agriculture and Rural Affairs,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]中国水产科学研究院长江水产研究所,农业农村部长江中上游渔业生态环境监测中心,武汉430223 [3]华中农业大学水产学院,武汉430070

出　　处：《淡水渔业》2020年第2期26-30,共5页Freshwater Fisheries

基　　金：中国水产科学研究院基本科研业务费资助(2017HY-ZD0206);公益性行业(农业)科研专项(201503108);国家自然科学基金青年科学基金项目(21507162);中国长江三峡集团公司科研项目(0799555)。

摘　　要：为探讨不同温度条件下十溴联苯醚(BDE-209)对鱼类的毒性影响,以草鱼(Ctenopharyngodon idellus)幼鱼离体肝脏组织为试验材料,在肝脏组织匀浆液中加入浓度为0.00、0.04、4.00、400.00μg/L的BDE-209,并将其分别置于低温(15℃)、常温(25℃)、高温(35℃)中水浴60 min,然后检测肝脏组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)的活性以及丙二醛(MDA)的含量。结果显示:低温时各浓度组BDE-209诱导下的SOD、CAT、GR活性与对照组没有显著性差异;常温时BDE-209对SOD、CAT、GR活性具有低浓度诱导高浓度抑制的现象,高温时BDE-209对肝脏组织中CAT、SOD、GR活性造成明显的抑制效应,MDA在不同温度条件下其含量都随BDE-209暴露浓度的升高而升高。研究结果表明:在一定的温度范围内,温度越高,BDE-209对鱼体肝脏组织造成的氧化损伤越大。In order to investigate the toxicity of decabromodiphenyl ether(BDE-209)to fish under different temperature conditions,the liver tissue of juvenile Ctenopharyngodon idellus were used in vitro study.The liver homogenate was added BDE-209 to the final concentration 0.00,0.04,4.00,400.00μg/L and incubated at 15℃,25℃,35℃for 60 min,respectively.The activities of superoxide dismutase(SOD),catalase(CAT),glutathione reductase(GR)and the content of malondialdehyde(MDA)in liver tissue were detected.The results showed that the activities of SOD,CAT and GR were not significant compared to the control group after exposure to BDE-209 at low temperature.The activities of SOD,CAT,GR were increased at low concentration and decreased at high concentration after exposure to BDE-209 at normal temperature.The activities of SOD,CAT and GR were significantly decreased after exposed to BDE-209 at high temperature.The MDA contents were increased along with BDE-209 doses at all temperature treated groups.The results showed that elevated temperature will enhance the oxidative damage of BDE-209 on liver tissue of fish within a certain temperature range.

关 键 词：草鱼(Ctenopharyngodon idellus) 温度 十溴联苯醚 毒性 

分 类 号：X503.225[环境科学与工程—环境工程]