二价钴离子对序批式反应器性能、微生物活性及其微生物群落的影响  

Effects of Divalent Cobalt (Co(Ⅱ)) on the Performance, Microbial Activity and Microbial Community of Sequencing Batch Reactor

作　　者：赵长坤 于娜玲 马丙瑞 李姗姗 信帅帅 武原原 潘云浩 吴淑妍 高孟春[1,2] ZHAO Chang-Kun;YU Na-Ling;MA Bing-Rui;LI Shan-Shan;XIN Shuai-Shuai;WU Yuan-Yuan;PAN Yun-Hao;WU Shu-Yan;GAO Meng-Chun(The Key Laboratory of Marine Environmental Science and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100,China;College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [2]中国海洋大学环境科学与工程学院,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2020年第6期101-108,共8页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(51178437)资助。

摘　　要：本文考察了二价钴离子(Co(Ⅱ))浓度变化对序批式反应器(SBR)性能、脱氮速率、微生物酶活性和微生物群落的影响。结果表明,进水中Co(Ⅱ)浓度在0~10 mg/L时,COD和NH+4-N去除率分别为(92.21±1.31)%和(98.40±0.66)%。在进水Co(Ⅱ)浓度为20 mg/L时,COD和NH+4-N去除率分别降至(81.78±0.52)%和(80.30±1.08)%。与进水未添加Co(Ⅱ)时相比,活性污泥比耗氧速率、脱氮速率、脱氢酶活性和与脱氮相关的微生物酶活性在进水Co(Ⅱ)浓度小于5 mg/L时略微升高,而在进水Co(Ⅱ)浓度为10和20 mg/L时则明显降低。活性污泥活性氧产生量和乳酸脱氢酶释放量随进水Co(Ⅱ)浓度升高而逐渐增加,表明Co(Ⅱ)的存在能造成细胞氧化应激和细胞膜损伤。随着进水Co(Ⅱ)浓度从0 mg/L升至20 mg/L,活性污泥微生物群落丰富度和多样性逐渐降低,且活性污泥中硝化菌属(Nitrosomonas、Nitrospira)和反硝化菌属(Luteimonas、Flavobacterium、Comamonas、Thauera和Zoogloea)的相对丰度发生改变,从而影响SBR脱氮性能。The performance,nitrogen removal rate,microbial enzymatic activity and microbial community of a sequencing batch reactor(SBR)were evaluated at different Co(Ⅱ)concentrations.The present results indicated that the COD and NH+4-N removal efficiency maintained at(92.21±1.31)%and(98.40±0.66)%at 0~10 mg/L Co(Ⅱ),respectively,whereas reduced to(81.78±0.52)%and(80.30±1.08)%at 20 mg/L Co(Ⅱ),respectively.Compared to the absence of Co(Ⅱ),the oxygen uptake rate,nitrogen removal rate,dehydrogenase activity and microbial enzymatic activity related to nitrogen removal had slight increase at below 5 mg/L Co(Ⅱ),whereas showed obvious decrease at 10 and 20 mg/L Co(Ⅱ).The reactive oxygen species production and lactate dehydrogenase release increased with the increase of Co(Ⅱ)concentration,suggesting that the presence Co(Ⅱ)caused oxidative stress and cytomembrane damage of activated sludge microorganisms.High throughput sequencing indicated that the microbial richness and diversity of activated sludge gradually decreased with Co(Ⅱ)concentration increasing from 0 to 20 mg/L.The relative abundance of nitrification genera(e.g.Nitrosomonas and Nitrospira)and denitrification genera(e.g.Luteimonas,Flavobacterium,Comamonas,Thauera and Zoogloea)changed under different Co(Ⅱ)concentrations,which could further affect the nitrogen removal of SBR.

关 键 词：Co(Ⅱ) 活性污泥 脱氮速率 微生物酶活性 微生物群落 

分 类 号：X703[环境科学与工程—环境工程] X172