JWH133对MPP^+诱导的原代星形胶质细胞COX-2和iNOS表达的影响  

EFFECT OF JWH133 ON MPP^+-INDUCED CYCLOOXYGENASE-2 AND INDUCIBLE NITRIC OXIDE SYNTHASE EXPRESSION IN PRIMARY CULTURED ASTROCYTES

作　　者：贾翼 邓晗 马泽刚[1] JIA Yi;DENG Han;MA Zegang(Department of Physiology, School of Basic Medicine, Qingdao University, Qingdao 266071, China)

机构地区：[1]青岛大学基础医学院生理学教研室,山东青岛266071

出　　处：《青岛大学学报（医学版）》2020年第2期156-160,共5页Journal of Qingdao University(Medical Sciences)

基　　金：山东省重点研发计划资助项目(2019GSF108095)。

摘　　要：目的探讨大麻素Ⅱ型受体(CB2受体)激活对1-甲基-4-苯基-吡啶离子(MPP+)诱导原代星形胶质细胞炎症反应的影响。方法培养原代星形胶质细胞,生长状态良好时将其分为Control组、MPP+组、JWH133(CB2受体激动剂)+MPP+组、AM630(CB2受体抑制剂)+JWH133+MPP+组。应用免疫印迹法(Western Blot)检测Control组和MPP+组细胞CB2受体蛋白的表达。应用实时荧光定量PCR(RT-PCR)检测各组环氧化酶2(COX-2)和诱导型一氧化氮合酶(iNOS)基因的表达。结果与Control组相比,MPP+组的CB2受体蛋白表达上升(F=29.78,P<0.01)。与Control组比较,MPP+组COX-2和iNOS基因的表达明显上调(F=22.59、11.27,q=10.13、5.57,P<0.01);JWH133预处理抑制MPP+诱导的COX-2和iNOS基因表达的上调(q=6.26、4.16,P<0.05);此抑制作用可被AM630所阻断(q=5.34、5.67,P<0.01)。结论激活CB2受体可抑制MPP+诱导的原代星形胶质细胞炎症反应。Objective To investigate the effect of cannabinoid 2 receptor(CB2 receptor)on 1-methyl-4-phenylpyridinium(MPP+)-induced inflammatory response in primary cultured astrocytes.Methods Primary cultured astrocytes were cultured and divided into Control group,MPP+group,JWH133(CB2 receptor agonist)+MPP+group,and AM630(CB2 receptor antagonist)+JWH133+MPP+group when growing well.Western blotting was used to determine the protein expression of CB2 receptor in Control group and MPP+group.Quantitative real-time PCR was performed to measure the mRNA expression of cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS).Results Compared with Control group,the MPP+group had significantly up-regulated CB2 receptor protein expression(F=29.78,P<0.01)and significantly up-regulated COX-2 and iNOS mRNA expression(F=22.59,11.27;q=10.13,5.57;P<0.01).JWH133 pre-treatment significantly inhibited the up-regulated mRNA expression of COX-2 and iNOS induced by MPP+(q=6.26,4.16;P<0.01),while this was blocked by CB2 receptor antagonist AM630(q=5.34,5.67;P<0.01).Conclusion Activation of CB2 receptor can inhibit MPP+-induced inflammatory response in primary cultured astrocytes.

关 键 词：受体 大麻酚 CB2 大麻素受体激动剂 1-甲基-4-苯基吡啶 星形细胞 环氧化酶2 一氧化氮合酶Ⅱ型 

分 类 号：R338.2[医药卫生—人体生理学]