Ghrelin对小鼠海马CA1区锥体神经元突触活动影响  

EFFECT OF GHRELIN ON SYNAPTIC ACTIVITY OF PYRAMIDAL NEURONS IN THE HIPPOCAMPAL CA1 REGION OF MICE

作　　者：徐凤华 李楠 张萌 史东萍 张天一 周宇 XU Fenghua;LI Nan;ZHANG Meng;SHI Dongping;ZHANG Tianyi;ZHOU Yu(Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao University, Qingdao 266071, China)

机构地区：[1]青岛大学医学部基础医学院生理学教研室,山东青岛266071

出　　处：《青岛大学学报（医学版）》2020年第2期165-168,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(31471079)。

摘　　要：目的探讨胃促生长素(ghrelin)对小鼠海马CA1区锥体神经元突触活动的影响。方法用12~20周龄小鼠制备脑薄片,运用全细胞膜片钳技术记录ghrelin对小鼠海马CA1区锥体神经元突触活动的影响。结果与仅灌流人工脑脊液(ACSF)对照组相比,200 nmol/L ghrelin灌流增加小鼠海马CA1区锥体神经元微小兴奋性突触后电流(mEPSCs)的幅度(t=2.141,P<0.05),而对CA1区锥体神经元mEPSCs的频率、锥体神经元自发兴奋性突触后电流(sEPSCs)的幅度和频率均无明显影响(P>0.05)。Ghrelin不影响海马CA1区抑制性突触后电流的频率和幅度(P>0.05)。结论200 nmol/L ghrelin可以提高小鼠海马CA1区锥体神经元mEPSCs的幅度,而对mEPSCs的频率无影响,提示其对兴奋性突触传递的调节作用可能主要是通过突触后机制实现的。Objective To investigate the effect of ghrelin on the synaptic activity of pyramidal neurons in the hippocampal CA1 region of mice.Methods Hippocampal slices were prepared from the mice aged 12-20 weeks,and the whole-cell patch clamp technique was used to evaluate the effect of ghrelin on the synaptic activity of pyramidal neurons in the hippocampal CA1 region of mice.Results Compared with the control group treated with artificial cerebrospinal fluid(ACSF)hemoperfusion,the group treated with ghrelin(200 nmol/L)hemoperfusion had a significant increase in the amplitude of miniature excitatory postsy-naptic currents(mEPSCs)in pyramidal neurons in the hippocampal CA1 region(t=2.141,P<0.05),with no significant changes in the frequency of mEPSCs and the amplitude and frequency of spontaneous excitatory postsynaptic currents(P>0.05).Ghrelin did not affect the frequency and amplitude of inhibitory postsynaptic currents in the hippocampal CA1 region(P>0.05).Conclusion Ghrelin(200 nmol/L)can increase the amplitude of mEPSCs in pyramidal neurons in the hippocampal CA1 region and has no effect on the frequency of mEPSCs,suggesting that ghrelin may regulate excitatory synaptic transmission through post-synaptic mechanism.

关 键 词：胃促生长素 膜片钳术 CA1区 海马 锥体细胞 兴奋性突触后电位 小鼠 

分 类 号：R338.2[医药卫生—人体生理学] R329.25[医药卫生—基础医学]