淫羊藿苷对原代星形胶质细胞炎症反应的抑制作用  

INHIBITORY EFFECT OF ICARIIN AGAINST INFLAMMATORY RESPONSE IN PRIMARY ASTROCYTES

作　　者：王皓田 李娜[1] 陈文芳[1] WANG Haotian;LI Na;CHEN Wenfang(Department of Physiology and Pathophysiology, School of Basic Medicine, Qingdao University, Qingdao 266071, China)

机构地区：[1]青岛大学基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2020年第2期181-184,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(31871144);山东省自然科学基金面上项目(ZR2019MH018)。

摘　　要：目的探讨淫羊藿苷(ICA)对脂多糖(LPS)诱导的原代星形胶质细胞炎症反应的抑制作用。方法将细胞种于6孔板,分为对照组、LPS组和ICA给药组。对照组先用1μL二甲基亚矾(DMSO)处理细胞1 h,再加入1μL生理盐水;LPS组先用1μL DMSO预处理细胞1 h,再加入LPS(终浓度1 mg/L)处理细胞6 h;ICA给药组先用不同浓度的ICA(0.1、1.0、10.0、20.0μmol/L)预处理细胞1 h,然后与LPS共同孵育细胞6 h。采用荧光定量PCR(RT-PCR)法分别检测各组细胞胶质纤维酸性蛋白(GFAP)mRNA的表达。应用RT-PCR法检测ICA给药组(10.0μmol/L)肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)mRNA的表达,并与对照组、LPS组检测结果比较。结果与对照组相比较,LPS组的GFAP mRNA表达明显升高(F=10.63,q=9.775,P<0.01);1.0、10.0、20.0μmol/L的ICA均能够明显抑制LPS诱导的GFAP mRNA的表达(q=4.496~6.111,P<0.05),以10.0μmol/L ICA的抑制效果最明显(q=6.111,P<0.01)。与对照组相比较,LPS组的TNF-α和IL-1βmRNA表达明显升高(F=243.50、378.60,q=30.040、36.000,P<0.01),10.0μmol/L ICA组TNF-α和IL-1βmRNA表达较LPS组显著降低(q=7.689、5.199,P<0.05)。结论ICA能够抑制LPS诱导的原代星形胶质细胞的活化和炎症反应。Objective To investigate the inhibitory effect of icariin(ICA)against lipopolysaccharide(LPS)-induced inflammatory response in primary astrocytes.Methods Cells were seeded in a 6-well plate and divided into control group,LPS group,and ICA groups.The control group was treated with 1μL DMSO for 1 h and then 1μL normal saline for 6 h.The LPS group was treated with 1μL DMSO for 1 h and then LPS(final concentration,1 mg/L)for 6 h.The ICA groups received 1 h pretreatment with ICA at 0.1,1.0,10.0,and 20.0μmol/L,respectively,followed by co-incubation with LPS for 6 h.RT-PCR was used to determine the mRNA expression of glial fibrillary acidic protein(GFAP)in all the groups,and to determine the mRNA expression of TNF-αand IL-1βin the control group,the LPS group,and the 10.0μmol/L ICA group.Results Compared with the control group,the LPS group showed a significantly higher mRNA expression level of GFAP(F=10.63,q=9.775,P<0.01);LPS-induced mRNA expression of GFAP was significantly inhibited by ICA at 1.0,10.0,and 20.0μmol/L(q=4.496-6.111,P<0.05),with the strongest inhibitory effect at 10μmol/L(q=6.111,P<0.01).The mRNA expression levels of TNF-αand IL-1βin the LPS group were significantly higher than those in the control group(F=243.50,378.60;q=30.040,36.000;P<0.01),and also significantly higher than those in the 10.0μmol/L ICA group(q=7.689,5.199;P<0.05).Conclusion ICA inhibits LPS-induced activation of primary astrocytes and inflammatory response.

关 键 词：淫羊藿甙 脂多糖 星形细胞 肿瘤坏死因子α 白细胞介素1Β 神经胶质原纤维酸性蛋白质 

分 类 号：Q421[生物学—神经生物学]