多脂大戟中Lathyrane-type类二萜化合物对烟草花叶病毒的抑制活性分析  

Inhibition Effects of Lathyrane-type Sesquiterpenoids from Euphorbia resinifera on Tobacco Mosaic Virus

作　　者：李博文 赵宁东 赵立华[2] 何思洁 张丽珍[2] 李顺林[4] 张洁[2] 郑雪[2] 龙琴 张仲凯[2] 李靖 LI Bo-wen;ZHAO Ning-dong;ZHAO Li-hua;HE Si-jie;ZHANG Li-zhen;LI Shun-lin;ZHANG Jie;ZHENG Xue;LONG Qin;ZHANG Zhong-kai;LI Jing(Life Science College,Southwest Forestry University,Yunnan Kunming 650224,China;Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Yunnan Provincial Key Laboratory of Agricultural Biotechnology,Key Lab of South­western Crop Gene Resource and Germplasm Innovation,Ministry of Agriculture,Yunnan Kunming 650223,China;College of Chemistry and Engineering,Wenshan University,Yunnan Wenshan 663000,China;Kunming Institute of Botany,Chinese Academy of Sciences,Yunnan Kunming 650201,China)

机构地区：[1]西南林业大学生命科学学院,云南昆明650224 [2]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业部西南作物基因资源与种质创制重点实验室,云南昆明650223 [3]文山学院化学与工程学院,云南文山663000 [4]中国科学院昆明植物研究所,云南昆明650201

出　　处：《西南农业学报》2020年第4期811-816,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31960531,31560499,31900300);云岭人才2015;云南省应用基础研究计划重点项目(2018FA020);云南省中青年学术技术带头人后备人才项目(2015HB081)。

摘　　要：【目的】烟草花叶病毒(Tobacco mosaic virus,TMV)严重危害烟草、番茄、辣椒等多种作物,给农业生产造成巨大经济损失,本研究为利用低毒、高效植物源药剂防治TMV病毒病害提供先导化合物和理论依据。【方法】采用枯斑计数法对从多脂大戟中分离的11种化合物进行抗TMV活性测定。【结果】接种TMV 24 h后施用100μg/mL的化合物制剂进行防控实验,其中化合物Euphorblin E(HZ-7)的抑制率为67.54%,高于对照宁南霉素(52.89%);先施用11种化合物制剂6 h后再接种TMV进行预防实验,其中化合物Euphorblin E(HZ-7)抑制率为72.29%,高于对照宁南霉素(58.21%)。【结论】多脂大戟中化合物Euphorblin E可作为抗TMV病毒病害环保型生物源农药研发的先导化合物。【Objective】Tobacco mosaic virus(TMV)infects tobacco,tomato,pepper and other cash crops and causes huge economic losses to agricultural production,antiviral lead compounds and theoretical basis were provided for controlling TMV virus disease by using low toxicity and high efficiency botanical agents.【Method】Lesion counting was used to detect the activities of TMV after treating with 11 sesquiterpenoids from Euphorbia resinifera.【Result】11 sesquiterpenoids(100μg/mL)were sprayed after being inoculated with TMV for 24 hours,the inhibition rate of Euphorblin E(HZ-7)was 67.54%,which was higher than that of the control Ningnanmycin(52.89%);inoculated with TMV after sprayed 11 sesquiterpenoids for 6 hours,the inhibition rate of Euphorblin E(HZ-7)was 72.29%,which was higher than that of the control Ningnanmycin(58.21%).【Conclusion】The compound Euphorblin E from Euphorbia resinifera could be used as a lead compound for the development of environment friendly pesticides against TMV.

关 键 词：烟草花叶病毒 多脂大戟 二萜 抑制率 烟草 

分 类 号：S463[农业科学—植物保护]