蝉花子实体抗疲劳作用研究  

Study of anti-fatigue effect for Cicada flower fruiting body

作　　者：孙长胜 陈桃宝 龙文君 李春如 周宏灏[1,2] 王玉芹 SUN Changsheng;CHEN Taobao;LONG Wenjun;LI Chunru;ZHOU Honghao;WANG Yuqin(Department of Clinical Pharmacology,Xiangya Hospital,Central South University,Changsha 410008,China;Hunan Key Laboratory of Pharmacogenetics,Changsha 410078,China;Zhejiang Bioasia Life Science Institute,Pinghu 314200,China;Zhejiang Bioasia Pharmaceutical Co.,Ltd.,Pinghu 314200,China)

机构地区：[1]中南大学湘雅医院临床药理研究所,湖南长沙410008 [2]遗传药理学湖南省重点实验室,湖南长沙410078 [3]浙江泛亚生命科学研究院,浙江平湖314200 [4]浙江泛亚生物医药股份有限公司,浙江平湖314200

出　　处：《药物评价研究》2020年第4期642-647,共6页Drug Evaluation Research

基　　金：国家星火计划(2015GA700011)。

摘　　要：目的研究蝉花子实体的抗疲劳、增强运动耐力作用。方法 ICR雄性小鼠随机分为对照组、人参乙醇提取物(阳性药,2.5 g/kg)组以及蝉花子实体低、中、高剂量(0.075、0.150、0.300 g/kg)组,连续ig给药15 d后,测定小鼠负重游泳力竭时间,试剂盒法检测小鼠经游泳运动后肝糖原、肌糖原、全血乳酸(LD)、血清尿素氮(BUN)、超氧化歧化酶(SOD)和乳酸脱氢酶(LDH)等与疲劳相关联的生化指标变化。结果与对照组比较,蝉花子实体低、中剂量对小鼠力竭游泳时间有显著延长作用(P<0.05),低剂量组LD水平显著下降(P<0.05),高剂量LDH含量显著升高(P<0.05),低剂量组SOD含量显著升高(P<0.05);中、高剂量组BUN含量显著降低(P<0.05),低剂量组肝糖原水平显著升高(P<0.05);中剂量组肌糖原水平显著升高(P<0.05)。结论蝉花子实体对运动后的小鼠有抗疲劳作用。Objective To study cicadae anti-fatigue and enhance exercise endurance effects. Methods ICR male mice were randomly divided into different groups, control group, the ginseng group, cicadae high, medium and low dose(0.075, 0.150, and0.300 g/kg) group, continuous ig administration of 15 d, different groups were measured by loading swimming time, liver glycogen,muscle glycogen, blood urea nitrogen(BUN), blood lactic acid(LD), superoxide dismutase(SOD) and lactate dehydrogenase(LDH), fatigue associated with changes in biochemical markers. Results Compared with the control group, the low and middle dose of Cicada flower body significantly prolonged the exhaustive swimming time of mice(P < 0.05), the level of LD in the low dose group significantly decreased(P < 0.05), the content of LDH in the high dose group significantly increased(P < 0.05), the content of SOD in the low dose group significantly increased(P < 0.05), the content of BUN in the middle and high dose groups significantly decreased(P < 0.05), and the level of liver glycogen in the low dose group significantly increased(P < 0.05). In the middle dose group, the level of muscle glycogen was significantly increased(P < 0.05). Conclusion Cicada flower fruiting body has anti-fatigue effect on mice after exercise.

关 键 词：蝉花子实体 抗疲劳 运动耐力 肝糖原 肌糖原 乳酸 超氧化歧化酶 

分 类 号：R285.5[医药卫生—中药学]