不同浓度七氟醚对急性心肌缺血再灌注老龄大鼠血清、心脏5-HT、白细胞介素和海马组织SOD、MDA的影响  

Effects of sevoflurane concentration on serum and heart 5-HT, IL-1α, IL-1β and SOD, MDA in hippocampal tissue of aged rats with acute myocardial ischemiareperfusion model

作　　者：尹彩星[1] 龚清安[1] 司小萌[1] YIN Caixing;GONG Qingan;SI Xiaomeng(Department of Anesthesiology,Nanyang Central Hospital,Nanyang 473000,China)

机构地区：[1]南阳市中心医院麻醉科,河南南阳473000

出　　处：《药物评价研究》2020年第4期700-705,共6页Drug Evaluation Research

基　　金：河南省科技发展计划(152300410163)。

摘　　要：目的探究七氟醚对急性心肌缺血再灌注(AIRI)模型的老龄大鼠血清5-羟色胺(5-HT)、白介素(IL)-1α、IL-1β和海马组织超氧化物歧化酶(SOD)、丙二醛(MDA)的影响。方法选取SPF级健康雄性老龄大鼠40只,随机分为5组,建立AIRI模型,模型组给予氧气吸入,七氟醚组分别吸入1%、2%、3%及4%的七氟醚,采用免疫组织化学法检测大鼠心脏组织5-HT、IL-1α、IL-1β的蛋白表达量;ELISA法检测血清5-HT、IL-1α、IL-1β浓度;采用黄嘌呤氧化酶法测定海马组织SOD的活性,硫代巴比妥酸显色法测定海马组织MDA活性;实时荧光定量PCR(RT-qPCR)法检测血清5-HT、IL-1α、IL-1β和海马组织SOD、MDA中mRNA的表达量。结果与模型组比较,七氟醚2%、3%、4%浓度组心脏组织、血清中5-HT、IL-1α、IL-1β蛋白水平、血清中5-HT、IL-1α、IL-1βmRNA水平显著降低(P<0.05);七氟醚2%、3%、4%浓度组海马组织MDA蛋白及mRNA水平显著降低,SOD蛋白及mRNA水平显著升高(P<0.05)。结论 2%~4%的七氟醚能够显著降低AIRI模型老龄大鼠心脏、血清5-HT、IL-1α、IL-1β表达水平,降低海马组织MDA水平,提高SOD活性。Objective To investigate the effects of sevoflurane concentration on serum 5-hydroxytryptamine(5-HT), interleukin(IL)and malondialdehyde(MDA) in hippocampal tissue of aged rats with acute myocardial ischemia-reperfusion(AIRI) model.Methods Totally 40 healthy male aged rats of SPF grade were randomly divided into five groups with eight rats in each group. The rats model of AIRI was established. Rats in control group was inhaled with oxygen, the sevoflurane group inhaled 1%, 2%, 3% and4% sevoflurane respectively. The protein expression of 5-HT, IL-1α and IL-1β in serum of rats inhaled with different concentrations of sevoflurane was detected by immunohistochemistry, and the concentration of sevoflurane was detected by ELISA. At the same time, the activity of SOD was determined by xanthine oxidase method and the activity of MDA was determined by thiobarbituric acid colorimetric method. The expression of mRNA of 5-HT, IL-1α and IL-1β and hippocampal SOD, MDA was detected by RTqPCR. Results Compared with model group, the levels of 5-HT, IL-1 α, IL-1 β protein and mRNA in the heart and serum of the group with 2%, 3% and 4% sevoflurane decreased significantly(P < 0.05);the levels of MDA protein and mRNA in the hippocampus of the group with 2%, 3% and 4% sevoflurane decreased significantly, and the levels of SOD protein and mRNA increased significantly(P < 0.05). Conclusion 2%-4% sevoflurane can significantly reduce the expression level of 5-HT, IL-1 α,IL-1β in the heart and serum of aging rats with AIRi model, reduce the MDA level in hippocampus and improve the activity of SOD.

关 键 词：七氟醚 浓度 急性心肌缺血再灌注 老龄大鼠 5-羟色胺 白细胞介素 超氧化物歧化酶 丙二醛 

分 类 号：R965.1[医药卫生—药理学]