去乙酰转移酶ZmHDA101与TPL/TPRs蛋白的互作研究  

Study of interaction between deacetyltransferase ZmHDA101 and TPL/TPRs

作　　者：于璐 白华[1] 庞茜[1] 朱岩 张康 邢继红[1] 董金皋[1] YU Lu;BAI Hua;PANG Xi;ZHU Yan;ZHANG Kang;XING Jihong;DONG Jingao(Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/Mycotoxin and Molecular Plant Pathology Laboratory,Hebei Agricultural University,Baoding 071000,China;Hebei Pesticide Development and Application Association,Shijiazhuang 050031,China)

机构地区：[1]河北农业大学,河北省植物生理与分子病理学重点实验室/真菌毒素与植物分子病理学实验室,河北保定071000 [2]河北省农药发展与应用协会,河北石家庄050031

出　　处：《河北农业大学学报》2020年第2期83-89,共7页Journal of Hebei Agricultural University

基　　金：粮食丰产增效科技创新专项(2016YFD0300704);国家自然科学基金项目(31901864);河北省自然科学基金项目(C2019204141).

摘　　要：ZmHDA101是玉米去乙酰转移酶家族的重要成员之一,其作用的分子机制尚未明确。为了明确ZmHDA101与TPL/TPRs蛋白之间的互作关系,本研究利用Gateway技术,构建了ZmHDA101、TPL/TPRs的酵母双杂交载体AD/BD-ZmHDA101、AD/BD-AtTPL、AD/BD-AtTPR1、AD/BD-AtTPR2、AD/BD-AtTPR3和AD/BD-AtTPR4;利用酵母双杂交技术,分析了ZmHDA101与TPL/TPRs蛋白之间的互作关系。结果发现,共同转化了ZmHDA101与TPL/TPRs组合的酵母均能在-Leu/-Trp、-Leu/-Trp/-His和-Leu/-Trp/-His+3-AT培养基上正常生长,而转化了对照组合的酵母不能在-Leu/-Trp/-His或-Leu/-Trp/-His+3-AT培养基上正常生长,表明ZmHDA101与AtTPL、AtTPR1、AtTPR2、AtTPR3和AtTPR4在酵母细胞中均能够直接互作。研究结果为阐明ZmHDA101在玉米生长发育和抵抗生物/非生物胁迫中的功能及其调控机制奠定了基础。ZmHDA101 is one of the important members of the corn deacetyltransferase family, and the molecular mechanism of ZmHDA101’s action has not yet been clarified. In order to clarify the interaction between ZmHDA101 and TPL/TPRs proteins, this study used Gateway technology to construct yeast two-hybrid vectors AD/BDZmHDA101, AD/BD-AtTPL, AD/BD-AtTPR1, AD/BD-AtTPR2, AD/BD-AtTPR3 and AD/BD-AtTPR4.The yeast two-hybrid technology was used to analyze the interaction between ZmHDA101 and TPL/TPRs proteins. The yeast colonies which were co-transformed with ZmHDA101 and TPL/TPRs combinations grew wellin-Leu/-Trp,-Leu/-Trp/-His and-Leu/-Trp/-His+3-AT media.However, the yeast colonies which were co-transformed with negative controls could not grow normally in-Leu/-Trp/-His or-Leu/-Trp/-His+3-AT media.These results indicated that Zm HDA101 interacts withAtTPL, AtTPR1, AtTPR2, AtTPR3 and AtTPR4 in yeast cells. The results laid a foundation for elucidating the function of ZmHDA101 in maize growth, development and resistance to biotic/abiotic stress as well as its regulatory mechanism.

关 键 词：玉米 ZmHDA101 TPL/TPRs 酵母双杂交 自激活 

分 类 号：S432.1[农业科学—植物病理学]