不同消毒剂对HBV核酸和表面抗原的作用效果研究  

Effect of Various Disinfectants against HBV DNA and HBV Surface Antigen

作　　者：邱丰[1] 周为民[1] 尹文娇[1] 周文亭[1] 曹经瑗[1] 毕胜利[1] 武桂珍[1] Qiu Feng;Zhou Weimin;Yin Wenjiao(Insitute of Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《医学研究杂志》2020年第5期69-72,共4页Journal of Medical Research

基　　金：“十三五”国家科技重大专项基金资助项目(2018ZX10734-401)。

摘　　要：目的评价实验室常用的消毒剂对乙型肝炎病毒(HBV)的核酸和表面抗原的作用效果。方法采用悬液法将不同消毒剂与HBV混合,通过酶联免疫吸附试验(ELISA)评价消毒剂对乙肝表面抗原(HBsAg)的作用效果,通过实时荧光定量PCR评价消毒剂对HBV DNA的作用效果。结果对于高浓度的HBV核酸和HBsAg,1%卫可(Virkon)处理1h可使HBV DNA和HBsAg变为阴性,含氯消毒剂和75%乙醇仅对低浓度HBV血清有效,而过氧化氢对所有浓度的HBV核酸和HBsAg效果都不明显。结论当实验室发生HBV阳性血清溅洒等生物安全事故时,采用20~40倍体积1%卫可处理1h较为适宜。Objective To evaluate the effect of laboratory used disinfectants against HBV DNA and HBsAg.Methods Viral suspension test was applied to the evaluation.HBsAg was detected by ELISA and HBV DNA was measured by real-time fluorescence PCR.Results For the sera with high concentration of HBV DNA and HBsAg,after 1 hour incubation with 1%Virkon,both HBV DNA and HBsAg turned negative.However,chlorinated disinfectant and 75%ethanol were merely effective against HBV sera with low concentration.Hydrogen peroxide had little effect towards all kinds of HBV sera.Conclusion The treatment by 20-40-fold volume of 1%Virkon for an hour was recommended when HBV positive sera splashed in the laboratory.

关 键 词：乙型肝炎病毒 消毒剂 酶联免疫吸附试验 实时荧光定量PCR 

分 类 号：R512.6[医药卫生—内科学]