药材南天竹的组织学鉴定  

Histomorphological Identification of Nandina domestica

作　　者：付小梅[1,2] 孙菡 吴志瑰 刘婧[1] 裴建国 余绪晨[1] 邵镔淇[1] 舒积成 FU Xiao-mei;SUN Han;WU Zhi-gui;LIU Jing;PEI Jian-guo;YU Xu-chen;SHAO Bin-qi;SHU Ji-cheng(School of Pharmacy,Jiangxi University of Traditional Chinese Medicine(TCM),Nanchang 330004,China;FAN Cuisheng Studio of National Famous TCM,Nanchang 330006,China)

机构地区：[1]江西中医药大学药学院,南昌330004 [2]范崔生全国名中医传承工作室,南昌330006

出　　处：《中国实验方剂学杂志》2020年第9期120-128,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81102788,81760721);江西省自然科学基金项目(20161BAB20519);江西中医药大学校级研究生创新专项资金项目(JZYC18S10);江西省研究生优质课程(中药鉴定学);江西中医药大学项目(JXSYLXK-ZHYAO026)。

摘　　要：目的:通过对南天竹的根、茎、叶、果实进行系统的形态组织学研究,为其真伪鉴定提供依据。方法:采用性状鉴定方法对南天竹各药用部位进行外观特征描述;并采用石蜡切片技术和粉末制片技术对各药用部位如根、茎、叶、花、果实等横切面和粉末的显微特征进行观察,绘制墨线图并拍照。结果:发现该原植物形态特征与本草描述一致;药材性状为根、茎、叶、花、果实类生药特点;根横切面无髓部;茎横切面中可见皮层有断续成环的纤维束及木质部内侧的帽状纤维束;叶横切面中栅栏组织较为宽广,主脉维管束周围的纤维束成环;叶柄横切面中纤维束间断排列成环;果实横切面中可见多层石细胞组成的环带;根和茎的粉末特征中主要含有含晶石细胞;叶粉末中可见晶鞘纤维和不定式气孔;花粉末中可见花粉粒,具3孔沟,外壁网状雕纹明显,有较多的网纹细胞;果实粉末中可见大量分枝状石细胞及草酸钙方晶。结论:上述形态与显微鉴别特征有鉴定意义,为南天竹的真伪鉴别、质量标准及资源开发利用提供了科学依据。Objective:Histomorphological study of the Nandinae Radix,Nandinae Caulis,Nandinae Folium,Nandinae Fructus were conducted to provide the basis for the identification of its authenticity and falsehood.Method:The origin and macroscopic identification were used to describe the plant morphology and the appearance characteristics of all medicinal parts.The microscopic characteristics of the medicinal parts,such as roots,stems,leaves,flowers,fruits,were observed and photographed by paraffin section and powder preparation techniques.Result:It was found that the morphological characteristics of the original plant were consistent with the descriptions in herbaceous books.There was no pith on the cross-section of roots.In the transverse section of stems,there were intermittent circular fiber bundles in the cortex and a cap-shaped fibrous bundle in the inner part of the xylem.In the transverse section of the leaves,the palisade tissue was wider and the fiber bundles around the main vascular bundles formed a ring.In the transverse section of petiole,the fiber bundles were arranged intermittently into rings.In the transverse section of fruit,multi-layered sclereids formed a ring in the mesocarp.The powder characteristics of root and stem mainly contained crystalliferous sclereids.There were crystal sheath fibers and stomatal infinitive in the leaf powder,and pollen grains in the flower powder,with 3-hole grooves,obvious reticulate engraving pattern in the outer wall and more reticulate cells.There were a large number of branched sclereids and calcium oxalate square crystals in the fruit powder.Conclusion:The abovementioned morphological and microscopic features have identification significance,and provide scientific basis for the authenticity identification,the quality standard and the utilization of resources of Nandina domestica.

关 键 词：南天竹 组织 来源鉴定 性状鉴定 显微鉴定 根 茎 叶 果实 

分 类 号：R284.1[医药卫生—中药学] R289[医药卫生—中医学]