基于β-catenin信号通路内热针治疗腰椎退行性病变机制研究  

Inner-heating acupuncture of lumbar degenerative lesions based on β-catenin signaling pathway

作　　者：庄金刚 张雄波 陈冬 程肖芳 温建民[2] 王圆圆 ZHUANG Jin-gang;ZHANG Xiong-bo;CHEN Dong;CHENG Xiao-fang;WEN Jian-min;WANG Yuan-yuan(Shenzhen Hospital of Beijing University of Traditional Chinese Medicine,Shenzhen518000,Guangdong,China;不详)

机构地区：[1]北京中医药大学深圳医院,广东深圳518000 [2]中国中医科学院望京医院,北京100102

出　　处：《中国骨伤》2020年第5期484-488,共5页China Journal of Orthopaedics and Traumatology

基　　金：深圳市龙岗区科技发展专项资金(编号:LGKCYLWS2018000048);深圳市医疗卫生三名工程项目(编号:SZSM201805089)。

摘　　要：目的:通过观察内热针对腰椎退行性病变大鼠椎间盘组织分泌型糖蛋白(Wnt1)、轴蛋白(Axin)及β-链蛋白(β-catenin)表达的影响,探讨内热针对腰椎退行性病变大鼠纤维环细胞凋亡的调节作用机制。方法:采用Sun等造模方法,将40只SD大鼠随机分为正常组、模型组、内热针组、针刺组,每组10只,除了正常组大鼠外,余均进行造模。选取双侧大肠俞进行内热针及针刺治疗。采用Western-bolt法检测大鼠椎间盘组织Wnt1、Axin及β-catenin的表达。结果:与模型组相比,内热针组和针刺组Wnt1和β-catenin表达均显著下降(P<0.05);内热针组大鼠纤维环Axin表达显著增加(P<0.05),针刺组Axin表达差异无统计学意义(P>0.05)。结论:内热针能下调腰椎退行性病变大鼠纤维环中Wnt1和β-catenin的表达,上调Axin的表达,这表示该机制可能通过调节β-catenin信号通路相关因子的表达水平来治疗腰椎退行性疾病。Objective:By observing the effect of inner-heating acupuncture on the expression of Wntl,Axin and β-catenin in the intervertebral disc of rats with lumbar degenerative disease and to explore the regulatory mechanism of inner-heating acupuncture on the apoptosis of annulus fibrosus cells in rats with lumbar degenerative disease.Methods:Forty SD rats were randomly divided into normal group,model group,inner-heating acupuncture group and acupuncture group with 10 rats in each group.Except for normal group,all rats were modeled.Bilateral Da Chang Shu were selected for inner-heating acupuncture and acupuncture treatment.Western-bolt assay was used to detect the expression of Wntl,Axin and β-catenin in rat intervertebral discs.Results:Compared with the model group,Wntl and β-catenin expression in annulus fibrosus cells of rats in the innerheating acupuncture group and the acupuncture group were significantly decreased(P<0.05);the expression of Axin in annulus fibrosus of rats in the inner-heating acupuncture group was significantly increased(P<0.05).There was no significant difference in the expression of Axin in annulus fibrosus of rats between acupuncture group and model group(P>0.05).Conclusion:Innerheating acupuncture can down-regulate the expression of Wntl and β-catenin and up-regulate the expression of Axin in annulus fibrosus of rats with lumbar degenerative desease.It is suggested that the mechanism may be to regulate the expression of related factors in the β-catenin signaling pathway,so as to achieve the goal of treating lumbar degenerative diseases.

关 键 词：Β-CATENIN信号通路 内热针 腰椎退行性病变 

分 类 号：R681.5[医药卫生—骨科学]