水提取法和仿生提取法研究菲牛蛭不同炮制品的体外抗凝活性  被引量:5

In Vitro Anticoagulant Activity of Different Processed Products of Poecilobdella manillensis by Water Extraction and Bionic Extraction

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作  者:钟苗 雷艳 谭赫 王雄飞[1] 侯觉文 谢海林 单宇 袁瑞娟[1] ZHONG Miao;LEI Yan;TAN He;WANG Xiong-fei;HOU Jue-wen;XIE Hai-lin;SHAN Yu;YUAN Rui-juan(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;GuangXi Fu Xin Yi Biological Technology Co.Ltd.,Nanning 530000,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China)

机构地区:[1]北京中医药大学中药学院,北京102488 [2]广西复鑫益生物科技有限公司,广西南宁530000 [3]中国医学科学院北京协和医学院药用植物研究所,北京100193

出  处:《中国现代中药》2020年第3期379-383,397,共6页Modern Chinese Medicine

基  金:北京市自然科学基金(7172130);北京市与中央在京高校共建项目(BJGJ1635)。

摘  要:目的:采取不同的提取方法研究菲牛蛭炮制前后抗凝活性变化。方法:分别采用水提取法及模拟胃肠道环境的仿生提取法提取菲牛蛭活体冻干品、清水吊干品、滑石粉烫制品、酒浸闷烘品中的活性成分,测定活化部分凝血酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)、抗凝血酶活性4个凝血指标,并采用Lorry法测定其蛋白含量,综合评价2种提取方法下的菲牛蛭及其炮制品的抗凝活性。结果:无论是水提取法,还是仿生提取法,APTT、PT、TT均显示炮制后抗凝活性降低,抗凝活性顺序为活体冻干品>清水吊干品>酒浸闷烘品>滑石粉烫制品,此结果与抗凝血酶活性及蛋白含量顺序一致。结论:高温炮制方法会降低菲牛蛭的抗凝活性。Objective:Different extraction methods were used to study the changes of anticoagulant activity before and after processing of Poecilobdella manillensis.Methods:bionic extraction method and water extraction were used respectively to extract the anticoagulant active components in Poecilobdella manillensis living lyophilized product,hanging dry products,talcum powder fried products and wine immersing-baked products.Activated partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),and antithrombin activity were selected as anticoagulant activity index to evaluate the anticoagulant activities of different processed Poecilobdella manillensis.The protein content in each extract of the processed products was determined by Lorry method.Results:Whether water extraction or biomimetic extraction,the results from APTT,PT and TT all showed that the anticoagulant activity decreases after processing.the anticoagulant activity sequence was as follows:living lyophilized product>hanging dry products>wine immersing-baked products>talcum powder fried products,which is consistent with the anti-thrombin activity and protein content of different processed products of the Poecilobdella manillensis.Conclusions:Therefore,the traditional processing method will reduce the anticoagulant activity of the Poecilobdella manillensis,and it is preferable to use the lyophilized product or the hanging dry products as the anticoagulant drug in clinical use.

关 键 词:菲牛蛭 炮制 仿生提取 抗凝活性 

分 类 号:R282.74[医药卫生—中药学] R283[医药卫生—中医学]

 

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