萤火素酶互补实验检测蛋白互作  

Luciferase Complementation Assay for Detecting Protein Interactions

作　　者：赵燕 周俭民[1] Yan Zhao;Jianmin Zhou(Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《植物学报》2020年第1期69-75,共7页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.31471741)。

摘　　要：蛋白质-蛋白质相互作用在真核生物的各项生命活动中发挥重要作用。与其它蛋白质互作研究技术相比,借助于烟草(Nicotiana benthamiana)瞬时表达系统的萤火素酶互补实验(LCA)具有简单、灵敏、可靠、高效和低背景等优点,并可轻松扩展为大规模蛋白质互作的筛选和验证研究。该文介绍了萤火素酶互补实验的具体操作过程,通过2种数据收集方法来定性并定量分析生物发光或发光强度,从而检测植物目标蛋白之间的相互作用。Protein-Protein interactions play important roles in various eukaryotic biological processes.Compared to other techniques measuring protein-protein interactions in plants,the Luciferase Complementation Assay(LCA),based on Agrobacterium-mediated transient expression in Nicotiana benthamiana,is a simple,sensitive,reliable,highly quantitative and low background method that can be easily scaled up for high-throughput interactome studies.Here,we describe a protocol that includes two alternative data collection methods to qualitative and quantitative analyse luminescence or luminous intensity to detect protein-protein interactions in plant cells.

关 键 词：萤火素酶互补实验 蛋白质相互作用 烟草瞬时表达 

分 类 号：Q943.2[生物学—植物学]