怀牛膝细胞悬浮培养条件的优化  

Optimization of Cell Suspension Culture Conditions of Achyranthes bidentata

作　　者：李萍[1,2] 董亚辉 李成龙 何雨龙 李明军 Ping Li;Yahui Dong;Chenglong Li;Yulong He;Mingjun Li(College of Life Science,Henan Normal University,Xinxiang 453007,China;Zhengzhou Shuqing Medical College,Zhengzhou 450064,China;Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province/Engineering Laboratory of Biotechnology for Green Medicinal Plant of Henan Province,Xinxiang 453007,China)

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]郑州澍青医学高等专科学校,郑州450064 [3]河南省道地中药材保育及利用工程技术研究中心/绿色药材生物技术河南省工程实验室,新乡453007

出　　处：《植物学报》2020年第1期90-95,共6页Chinese Bulletin of Botany

基　　金：国家中医药行业科研专项子课题(No.201407005-08);河南省创新型科技人才队伍建设工程(No.C20130037);河南省高等学校青年骨干教师培养计划(No.2018GGJS293)。

摘　　要：为进一步优化怀牛膝(Achyranthes bidentata)细胞悬浮培养条件,对接种量、继代周期、pH、光照及Cu2+等多种影响因子的作用效果进行了研究,以提高怀牛膝细胞生长量及牛膝多糖含量。结果显示,接种量50 g·L^–1、继代周期14天,pH5–6和光照培养可以使细胞保持良好的生长状态及多糖合成能力;添加50μmol·L^–1 Cu^2+,细胞的干重最大,可达44.63 g·L^–1,多糖含量也最高,为4.02 mg·g^–1。To optimize the suspension culture conditions of Achyranthes bidentata cells,effect of different factors like inoculum concentrations,subculture cycles,pH,light and Cu2+on cell growth and polysaccharides contents was studied.The results showed that,cells grew well with a good ability for polysaccharides synthesis under 50 g·L^–1 inoculum concentration,14 d subculture cycle,pH5–6 and light.In the presence of 50μmol·L^–1 Cu^2+,the cell dry weight could be the maximum with 44.63 g·L^–1,and polysaccharides contents reached the peak at 4.02 mg·g^–1.

关 键 词：怀牛膝 细胞悬浮培养 细胞生长 多糖 

分 类 号：S567.239[农业科学—中草药栽培]