柯萨奇病毒A组6型对长爪沙鼠的感染性研究  

Study of coxsackievirus A6 infection in Mongolian gerbils

作　　者：高孟[1] 高丽美[1] 吴洁[1] 李剑波[1] 周冬 杨宏宏 罗永能[1] Gao Meng;Gao Limei;Wu Jie;Li Jianbo;Zhou Dong;Yang Hongkong;Luo Yongneng(Institute of Viral Diseases,Zhejiang Academy of Medical Sciences^Hangzhou 310053,China)

机构地区：[1]浙江省医学科学院病毒病研究所,杭州310013

出　　处：《中华实验和临床病毒学杂志》2019年第6期561-565,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：浙江省医学生物工程疫苗研发重点实验室(2008F3022);浙江省科技计划项目(2017C37120);浙江省重点研发计划项目(2018C03070)。

摘　　要：目的 研究柯萨奇病毒A组6型(coxsackievirus A group 6 type, CV-A6)对长爪沙鼠的感染性。方法 选取5个不同日龄组的长爪沙鼠,腹腔接种1×105 TCID50/只CV-A6 XS45株进行攻毒试验,观察各组病态症状,确定长爪沙鼠合适的攻毒日龄;而后选取合适日龄长爪沙鼠用3个剂量进行攻毒,观察剂量效应关系;攻毒长爪沙鼠病理组织(脑、肌肉、肠道)的病毒载量情况用荧光定量RT-PCR进行检测,组织感染病变情况用免疫组化进行检测。结果 1×105 TCID50/只的攻毒量CV-A6可造成7~35日龄长爪沙鼠100%发病、死亡,攻毒长瓜沙鼠症状评分变化趋势与鼠龄呈正相关;1×103 TCID50/只、1×104 TCID50/只、1×105 TCID50/只剂量组攻毒均可引起28日龄长爪沙鼠100%发病、死亡,攻毒长爪沙鼠症状评分变化趋势与攻毒剂量呈负相关;攻毒长爪沙鼠脑、肌肉、肠道组织均有不同程度的病毒载量,其中肌肉中的病毒载量高于其他组织,且组织中均发现病毒感染后细胞病变区域。结论 长爪沙鼠对CV-A6有很强的发病及死亡敏感性,可以作为理想的动物感染模型。Objective To study infection of coxsackievirus A6(CV-A6)in Mongolian gerbils.Methods To screen the optimal ages of Mongolian gerbils,five groups with different ages were infected with 1×105 TCID50 dose of CV-A6 XS45 strain by intraperitoneal,and symptom scores of Mongolian gerbils were collected.Then to estimate the dose-effect,three doses of virus were injected to the Mongolian gerbils.Quantitative reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemistry(IHC)were used to determine virus load and tissues infection in muscle,brain and intestinal tract.Results Mongolian gerbils infected with 1×105 TCID50 dose CV-A6 consistently exhibited clinical signs,and the morbidity(death)rates of five age groups were up to 100%.There was a positive correlation between the trend of symptom scores changes and ages.The morbidity(death)rates of three doses(1×103 TCID50,1×104 TCID50,1×105 TCID50)also were up to 100%in 28 days Mongolian gerbils.The correlation between the trend of symptom scores changes and doses were negative.Virus loads were detected in muscle,brain and intestinal tract of pathogenesis animal.The virus loads of muscle were higher than others.IHC results showed virus infection and cytopathic effects in three tissues.Conclusions Mongolian gerbils had high susceptibility to CV-A6,and were best for animal model of CV-A6 infection.

关 键 词：柯萨奇病毒A组6型 长爪沙鼠 感染模型 

分 类 号：R373.23[医药卫生—病原生物学] R-332[医药卫生—基础医学]