2018-2019年四川省A(H1N1)pdm09流感病毒抗原性及基因特性分析  

Antigenic and genetic characteristics of influenza A(H lNl)pdm 09 virus isolated in Sichuan province from 2018 to 2019

作　　者：杨慧萍[1] 徐佳楠[1] 童文彬[1] 刘李[1] 马小珍[1] 潘明[1] 李天舒[1] Yang Huiping;Xu Jianan;Tong Wenbin;Liu Li;Ma Xinozhen;Pan Ming;Li Tianshu(Microbiology Laboratory,Sichuan Provincial Center fo r Disease Control and Prevention,Chengdu 610041,China)

机构地区：[1]四川省疾病预防控制中心微生物检验所,成都610041

出　　处：《中华实验和临床病毒学杂志》2019年第6期580-585,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科技重大专项(2017ZX10103010-006)。

摘　　要：目的 了解2018-2019监测年度四川省人群A(H1N1)pdm09流感病毒的流行特点,明确当前流行株和疫苗株及国内外代表株的匹配情况及其抗原性和基因特性变异情况。方法 选取四川省流感网络实验室于2018年4月至2019年3月分离的A(H1N1)pdm09流感病毒株118株,利用红细胞凝集抑制试验进行抗原分析。并对其中16株低反应株的血凝素(hemagglutinin,HA)和神经氨酸酶(neuraminidase,NA)基因进行序列测定,采用BioEdit和MEGA5.0软件进行系统进化分析及氨基酸位点变异分析。结果 检测的95%以上的A(H1N1)pdm09流感病毒抗原性与疫苗株A/Michigan/45/2 015类似。HA基因特性分析显示,与疫苗株相比的4株低反应株分别在HA蛋白的Sa区、Sb区和Cb区发生了各一处氨基酸位点变异。15株低反应株均属6B.1分支,与国内外同期流行的代表株A/beijin-xicheng/SWL1633/2018和A/brisbane/02/2018进化关系最近。与A/sichuan/1/2009相比,在抗原决定区域(Sa区、Sb区、Ca和Cb区)和非决定区域分别发生了共6个和14个氨基酸位点共有突变。16株低反应株均在受体结合部位190螺旋发生了1处氨基酸位点变异。NA基因上耐药相关的氨基酸位点未发生变异。结论 2018-2019监测年度在四川省流行的A(H1N1)pdm09流感病毒与国内外流行特征一致,与WHO推荐的北半球的疫苗组分匹配。与2009年在国内首次分离的A(H1N1)pdm09流感病毒株A/sichuan/1/2009相比,在HA蛋白的抗原决定区和受体结合位点及NA蛋白的跨膜区、药物和抗体结合位点均发生基因变异。Objective To understand the epidemiological characteristics of influenza A(H1N1)pdm09 virus in Sichuan population during the monitoring period of 2018-2019,and to clarify the antigenic variation,the gene characteristics and the matching of current epidemic strains,vaccine strains,representative strains at home and abroad.Methods A total of 118 strains of influenza A(H1N1)pdm09 virus isolated in Sichuan region influenza network laboratory from April 2018 to March 2019 were selected.The hemagglutination inhibition(HI)assay was conducted for antigen analysis.The HA and NA genes of 16 strains with low-response strains were sequenced.Phylogenetic analysis and amino acid locus variation analysis were applied using BioEdit and MEGA5.0 software.Results The result of the antigen analysis demonstrated that more than 95%of the A(H1N1)pdm09 influenza viruses detected were similar to the WHO recommended vaccine strain A/Michigan/45/2015.The analysis of HA gene characteristics showed that some low-response strains had amino acid site variation in the Sa,Sb and Cb regions of the HA protein.A total of 15 low-response strains belonged to the 6B.1 branch.And their evolutionary relationship were close to the representative strains A/beijin-xicheng/SWL1633/2018 and A/brisbane/02/2018,which were popular at home and abroad.Compared with A/sichuan/1/2009,there are mutations involving 6,14 and 1 amino acid sites in the antigen-determining regions(Sa,Sb,Ca and Cb),non-determined regions and receptor binding site(RBS)respectively.No amino acid site mutations related to resistance to NA gene were found.Conclusions In 2018-2019,the epidemic A(H1N1)pdm09 influenza virus in Sichuan is consistent with the global epidemic characteristics,which also matched with vaccine strains recommended by WHO in the northern hemisphere.Compared with A/sichuan/1/2009 which was the first isolated in China in 2009,there were amino acid sites mutations in antigen-determining region and receptor binding site of the HA protein,and the transmembrane region of the N

关 键 词：A(H1N1)pdm09流感病毒 抗原性 基因特性 

分 类 号：R511.7[医药卫生—内科学] R181.3[医药卫生—临床医学]