2012-2017年甘肃省感染儿童中呼吸道合胞病毒的分子流行特征  

Molecular epidemiological characteristics of respiratory syncytial virus in pediatric patients in Gansu province from 2012 to 2017

作　　者：乔瑞娟[1] 陈建华[1] 武海卓 张燕[2] 张慧[1] 汪鹏[1] 张入学[3] 李宇宁[4] 高强 魏克敏[6] 于德山[1] Qiao Ruijuan;Chen Jianhua;Wu Haizhuo;Zhang Yan;Zhang Hui;Wang Pengx;Zhang Ruxue;Li Ylining;Gao Qiang;Wei Kemin;Yu Deshan(Gansu Center for Disease Control and Prevention,Lanzhou 730000,China;N ational Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Baiyin Center for Disease Control and Prevention,Baiyin 730900,China;Department of Pediatrics,the first Hospital of Lanzhou University y Lanzhou 730000,China;Longnan Center for Disease Control and Prevention,Longnan 746000,China;Tianshui Center for Disease Control and Prevention,Tianshui 741000,China)

机构地区：[1]甘肃省疾病预防控制中心,兰州730000 [2]中国疾病预防控制中心病毒病预防控制所,北京102206 [3]白银市疾病预防控制中心,730900 [4]兰州大学第一医院儿科,730000 [5]陇南市疾病预防控制中心,746000 [6]天水市疾病预防控制中心,741000

出　　处：《中华实验和临床病毒学杂志》2019年第6期586-592,共7页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科技重大专项(2017ZX10103006,2017ZX10104001);2015年甘肃省卫生行业科研计划(GSWSKY-2015-36)。

摘　　要：目的 了解甘肃省感染儿童中呼吸道合胞病毒(respiratory syncytial virus, RSV)的基因分型和变异变迁情况,掌握RSV的分子流行特征,预测RSV的流行型别与基因型。方法 2012-2017年从甘肃兰州、白银、张掖、天水、陇南等5个市哨点医院共收集到4 556份10岁以下急性呼吸道感染住院患儿的标本。使用实时荧光PCR方法检测获得RSV阳性的标本。再使用RT-PCR方法扩增RSV阳性标本的G蛋白编码基因全长进行测序,用DNAStar和MEGA 6.0等生物信息学软件进行序列拼接、比对、基因亲缘性和氨基酸变异特点等分析。结果 荧光PCR法共检测出1 135份RSV阳性标本,总体阳性率为24.91%,其中共获得216条G蛋白序列;根据G蛋白第二高变区的靶基因特征分析,甘肃省2012-2017年间RSV A亚型流行基因型包括NA1、NA3和ON1,G蛋白编码全长序列核苷酸同源性为84.9%~100%,氨基酸同源性为77.3%~100%,与原型株long株的核苷酸和氨基酸同源性分别为81.2%~83.3%和74.1%~88.0%。RSV B亚型流行基因型为BA9,G蛋白编码全长序列核苷酸同源性为97.7%~100%,氨基酸同源性为95.8%~100%,与原型株CH18 537株的核苷酸和氨基酸同源性分别为84.9%~85.7%和77.9%~80.1%。结论 本研究系统分析了2012-2017年甘肃省5市RSV A、B亚型G蛋白编码基因特征及氨基酸变异情况,为RSV病原研究以及感染控制提供依据。Objective To analyze the genotypes,amino acid vatiations and molecular epidemiological characteristics of respiratory syncytial virus(RSV)infection in pediatric patients in Gansu province for the future research.Methods A total of 4556 respiratory tract specimens were colleted from pediatric patients under 10 years of age in five cities in Gansu from 2012 to 2017.These specimens were tested for RSV and its subtypes.The coding region of the RSV G gene was amplified using reverse transcription-polymerase chain reaction(RT-PCR)and sequenced for RSV positive specimens.Sequences were edited using DNA Star software.Sequence alignment and phylogenetic trees were built by MEGA 6.0 software.Results Out of 4556 specimens,1135(24.91%)were positive for RSV,totally 216 G protein sequences were obtained.RSV A isolates were clustered into three genotypes:NA1、NA3 and ON1.The nucleotides and amino-acid homology was 84.9%-100%and 77.3%-100%,respectively.The nucleotides and amino-acid homology between this study and prototype long strain was 81.2%-83.3%and 74.1%-88.0%.RSV B isolates were clustered into only BA9 one genotypes.The nucleotides and amino-acid homology was 97.7%-100%and 95.8%-100%,respectively.The nucleotides and amino-acid homology between this study and prototype CH18537 strain was 84.9%-85.7%and 77.9%-80.1%.Conclusions The genetic characteristics and the amino-acid changes were analyzed systematically using data of RSV G gene collected from 2012 to 2017 in Gansu province in this study.These data were used for analyses of the etiology,control and prevention of RSV infection.

关 键 词：呼吸道合胞病毒 G蛋白 基因特征 分子流行 

分 类 号：R181.3[医药卫生—流行病学] R725.6[医药卫生—公共卫生与预防医学]