吴茱萸碱通过TLR4/NF-κB通路抑制脂多糖诱导的人脐静脉内皮细胞损伤  

Evodiamine inhibits injury of HUVECs induced by lipopolysaccharide through TLR4/NF-κB signaling pathway

作　　者：王嫣 杨卫平 彭芳 陈天琪 付云燕 田敏 WANG Yan;YANG Weiping;PENG Fang;CHEN Tianqi;FU Yunyan;TIAN Min(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025

出　　处：《细胞与分子免疫学杂志》2019年第12期1088-1093,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY2017-006);贵州省科技计划项目(ZY[2011]3010)。

摘　　要：目的观察吴茱萸碱对脂多糖(LPS)诱导人脐静脉内皮细胞(HUVEC)损伤的作用及机制。方法用HUVEC制备细胞炎症损伤模型,分为正常对照组、DMSO对照组、LPS模型组、吴茱萸低、高剂量组。采用CCK-8法检测细胞增殖能力,ELISA检测培养液上清中一氧化氮(NO)含量,反转录PCR检测细胞核因子κBp65(NF-κBp65)的mRNA水平,Western blot法检测细胞Toll样受体4(TLR4)、NF-κBp65的蛋白水平。结果与正常对照组相比,LPS模型组细胞的增殖能力显著降低,培养液中NO的含量显著降低,TLR4、NF-κBp65蛋白含量均明显增加。用吴茱萸碱预处理后,与模型组相比,细胞的增殖能力明显提高,培养液中NO的含量明显增加,同时细胞中TLR4、NF-κBp65蛋白的表达受到抑制,NF-κBp65 mRNA表达显著降低。结论吴茱萸碱可减轻LPS所致的内皮细胞炎性损伤,促进NO的合成,可能其与调控TLR4/NF-κB通路有关。Objective To observe the effect of evodiamine on the injury of human umbilical vein endothelial cells(HUVECs) induced by lipopolysaccharide(LPS) and investigate its impossible mechanism. Methods HUVECs were used to make the inflammatory injured cell model. The cultured cells in vitro were divided into a normal control group, LPS model group, DMSO control group, low-and high-dose evodiamine group. The cell survival rate was detected by CCK-8 assay. The content of nitric oxide(NO) in the medium serum was detected by ELISA. Nuclear factor-kappa Bp65(NF-κBp65) mRNA was determined by reverse transcription polymerase chain reaction(RT-PCR). The protein expression of Toll-like receptor 4(TLR4) and NF-κBp65 was detected by Western blotting. Results Compared with the control group, the cell survival rate and NO content obviously decreased, and the expression levels of TLR4 and NF-κBp65 increased in the LPS treated group. Compared with LPS group, the pre-treatment of evodiamine obviously improved the cell survival rate, promoted the secretion of NO, inhibited the expression of TLR4 and NF-κBp65 and down-regulated the mRNA expression of NF-κBp65. Conclusion Evodiamine can relieve the inflammatory injury of HUVECs induced by LPS and promote the synthesis of NO, which may be related to the regulation of TLR4/NF-κB signaling pathway.

关 键 词：吴茱萸碱 人脐静脉内皮细胞(HUVEC) Toll样受体4(TLR4) 核因子κB(NF-κB) 

分 类 号：R282[医药卫生—中药学] R714.252[医药卫生—中医学]