膝关节骨关节炎患者外周血NK细胞数量增加且表型异常  

Increased proportion and abnormal phenotype of NK cells in peripheral blood of patients with knee osteoarthritis

作　　者：彭志伟 徐斌[1] 王高远[1] 徐洪港[1] 张瀚元[1] 朱云[2] 徐龙 PENG Zhiwei;XU Bin;WANG Gaoyuan;Xu Honggang;ZHANG Hanyuan;ZHU Yun;XU Long(Sports Trauma and Arthroscopy,School of Basic Medicine,Anhui Medical University,Hefei 230022,China;Department of Oncology,First Affiliated Hospital,School of Basic Medicine,Anhui Medical University,Hefei 230022,China;Department of Immunology,School of Basic Medicine,Anhui Medical University,Hefei 230022,China)

机构地区：[1]安徽医科大学第一附属医院运动创伤与关节镜外科,安徽合肥230022 [2]安徽医科大学第一附属医院肿瘤内科,安徽合肥230022 [3]安徽医科大学基础医学院免疫学教研室,安徽合肥230022

出　　处：《细胞与分子免疫学杂志》2019年第12期1115-1121,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：安徽省自然科学基金(1808085MH243)。

摘　　要：目的探讨自然杀伤(NK)细胞在膝关节骨关节炎(KOA)外周血中的分布及表型特征。方法采用流式细胞术检测并分析对比无KOA的健康人群与KOA患者外周血中不同表型的NK细胞分布及其表面功能受体人类白细胞抗原DR(HLA-DR)、自然胞毒受体3(NCR3/NKP30)的表达情况。结果NK细胞在KOA患者外周血比例明显上调,其中以CD16bright NK细胞升高为主。KOA的NK细胞表面HLA-DR分子表达上调,而NKP30表达明显降低。结论NK细胞在KOA患者外周血中的比例增加,CD16、HLA-DR表达上调、NKP30表达下调。Objective To investigate the distribution and phenotypic characteristics of natural killer(NK) cells in the peripheral blood of knee osteoarthritis(KOA) patients. Methods Flow cytometry was used to detect and compare the distribution of NK cells and the expression of their surface functional receptors in the peripheral blood of healthy controls without KOA and patients with KOA, such as human leukocyte antigen DR(HLA-DR) and natural cytotoxicity receptor 3(NCR3/NKP30). Results The proportion of NK cells in the peripheral blood of KOA patients was significantly up-regulated, especially CD16bright NK cells. The expression of HLA-DR molecules on the surface of NK cells with KOA was up-regulated, while the expression of NKP30 was significantly down-regulated. Conclusion The proportion of NK cells in the peripheral blood of KOA patients increases, and the cells show the up-regulated expression of CD16 and HLA-DR and the down-regulated expression of NKP30.

关 键 词：膝关节 骨关节炎(OA) 自然杀伤细胞 流式细胞术 

分 类 号：R684.3[医药卫生—骨科学] R392.12[医药卫生—外科学]