Effects of "Candidatus Liberibacter solanacearum"(haplotype B)on Bactericera cockerelli fitness and vitellogenesis  

作　　者：Angelica Albuquerque Tomilhero Frias Freddy Ibanez Azucena Mendoza William Mario de Carvalho Nunes Cecilia Tamborindeguy 

机构地区：[1]Department of Entomology,Texas A&M University,412 Heep Center,College Station,Texas,USA [2]DEM-Depto.de Agronomia,Nucleo de Pesquisa em Biotecnologia Aplicada,Maringa,Brazil

出　　处：《Insect Science》2020年第1期58-68,共11页昆虫科学（英文版）

基　　金：supported by U.S.Department of Agriculture-National Institute of Food and Agriculture(USDA-NIFA)2012-67013-19431;Texas A&M University and Texas A&M AgriLife Research(Controlling Exotic and Invasive Insect-Transmitted Pathogens).Angelica Albuquerque Tomilhero Frias was supported by a scholarship from the PDSE/CAPES-Brazilian Federal Agency for Support and Evaluation of Graduate Education within the Ministry of Education of Brazil(Process:PDSE 88881.131726/2016-01).

摘　　要："Candidatus Liberibacter solanacearum"(Lso)are phloem-restricted and unculturable Gram-negative bacteria.Presently five haplotypes have been identified worldwide;but only haplotypes A and B are associated with the vector Bactericera cockerelli(Sulc.)in the Americas.Previous studies showed that Lso-infection reduces B.cockerelli reproductive output and that Lso haplotype B is more pathogenic than Lso haplotype A.To understand the interaction of Lso haplotype B and B.cockerelli,the fitness of Lso-free and Lso B-infected insects,and the expression of vitellogenin(5cKg7-like),a gene involved directly in the insect reproduction were analyzed.Statistical differences in the number of eggs oviposited,and the total number of progeny nymphs and adults were found among crosses of insects with or without Lso.Significant differences in sex proportions were found between Lso B-infected and Lso-free crosses:a higher proportion of Fi adult females were obtained from Lso B-infected mothers.A significant reduction of BcVgl-like was observed in crosses performed with Lso B-infected females compared to the Lso-free insects.In female cohorts of different age,a significant reduction of BcVgl-like expression was measured in 7-d-old Lso B-infected females(virgin and mated)compared with 7-d-old Lso-free females(virgin and mated),respectively.The reduction of BcVgl-like transcript was associated with a lower number of developing oocytes observed in female5s reproductive systems.Overall,this study represents the first step to understand the interaction of Lso B with B.cockerelli,highlighting the effect of Lso B infection on egg production,BcVgl-like expression,and oocyte development.

关 键 词：FEMINIZATION insect-pathogen interaction OVIPOSITION psyllids reproduction VITELLOGENESIS 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]