不同细胞数的卵裂期胚胎囊胚培养及冻融后单囊胚移植结局  

Blastocyst culture of different cell number from cleavage embryos and pregnancy outcome of their single frozen-thawed blastocyst

作　　者：戢开丽[1] 黎淑贞[1] 娄娟[1] 李云 郭江华[1] Ji Kaili;Li Shuzhen;Lou Juan;Li Yun;Guo Jianghua(Center for Assisted Reproduction, Jiangmen Central Hospital, Jiangmen, Guangdong 529030)

机构地区：[1]广东省江门市中心医院生殖医学中心,广东江门529030

出　　处：《中外女性健康研究》2020年第5期25-27,42,共4页Women＇s Health Research

摘　　要：目的:探索卵裂期胚胎细胞数对囊胚发育的影响,以及对冻融后单囊胚移植结局的影响。方法:回顾性选择D3胚胎评分A和B级5754枚行囊胚培养的胚胎,分为三组A组7~9细胞;B组≥10细胞;C组4~6细胞。比较三组的囊胚培养情况。选择177个单囊胚解冻移植周期,按照囊胚来源的D3胚胎评分A和B级分为两组A组7~9细胞和B组≥10细胞,比较冻融后单囊胚移植的结局。结果:三组胚胎囊胚培养结局比较,囊胚形成率、优质囊胚率、D5囊胚形成率和D5优质囊胚率差异极显著(P<0.01),但B组与A组比较,囊胚形成率、优质囊胚率差异不显著(P>0.05),D5囊胚形成率差异极显著(P<0.01),D5优质囊胚率差异显著(P<0.05)。冻融后单囊胚移植,两组D5囊胚比率和临床妊娠率差异不显著(P>0.05),但两组女方年龄差异极显著(P<0.01)。结论:在排除碎片的影响后,囊胚发育与D3胚胎细胞数有关,4~6细胞的囊胚形成率最低,而≥10细胞与7~9细胞相比则无差异;冻融后单囊胚移植虽然≥10细胞与7~9细胞比较临床妊娠率无显著差别,但未排除女方年龄的差异。Objective:To investigate the influence of cell number from cleavage embryos for blastocyst development,and to know pregnancy outcome of their single frozen-thawed blastocyst.Methods:This study retrospectively analyzed 5754 embryos for blastocyst culture were classified into three groups from cell number of day 3(D3)cleavage embryo scoring grade A and grade B:7~9 cells in A group;≥10 cells in B group;4~6 cell in C group.Then blastula formation was compared among the three groups.And according to cell number of D3 cleavage embryo,177 cycles frozen embryo transfer(FET)of single blastocyst from were classified two groups:7~9 cells in A group and≥10 cells in B group.Then pregnancy outcome was compared between the two groups.Results:There was extremely significant difference in blastocyst formation rate,high-quality blastocyst rate,day 5(D5)blastocyst formation rate,and D5 high-quality blastocyst rate among three groups(P<0.01),but no difference in blastocyst formation rate and high-quality blastocyst rate,and greater difference in D5 blastocyst formation rate and significant difference in D5 high-quality blastocyst rate between B group and A group.Moreover,clinical pregnancy and D5 blastocyst rate of single blastocyst on FET had no difference between A group and B group,but female age was greater different.Conclusion:Minimizing the impact of embryonic fragmentation,there was some correlation between blastocyst development and cell number of D3 embryo,blastocyst formation rate was lowest from 4~6 cells.There was no difference in blastocyst formation rate between≥10 cells and 7~9 cells.Clinical pregnancy of single blastocyst on FET had no significant difference between≥10 cells and 7~9 cells,but no excluding female age factor.

关 键 词：体外受精 囊胚培养 D3胚胎评分 细胞数 单囊胚移植 

分 类 号：R71[医药卫生—妇产科学]