罗非鱼循环水养殖系统中一株肺炎克雷伯氏菌的分离鉴定及其脱氮性能研究  

Identification and denitrificaition efficiency research on a strain of Klebsiella pneumoniae isolated from a tilapia recirculating aquaculture system

作　　者：王秀华[1] 武和英 杨冰[1] 张琴 于党辉 黄倢 WANG Xiu-hua;WU He-ying;YANG Bing;ZHANG Qin;YU Dang-hui;HUANG Jie(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Functional Laboratory of Marine Fisheries Science and Food Production Process,Qingdao National Laboratory for Marine Science and Technology,Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Key Laboratory of Maricultural Epidemiology and Biosecurity of Qingdao,Qingdao 266071,Shandong,China;Key Laboratory of Marine Biotechnology of Guangxi,Guangxi Institute of Oceanology,Beihai 536000,Guangxi,China)

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室,农业农村部海水养殖病害防治重点实验室,青岛市海水养殖流行病学与生物安保重点实验室,山东青岛266071 [2]广西壮族自治区海洋研究所,广西海洋生物技术重点实验室,广西北海536000

出　　处：《淡水渔业》2020年第3期72-78,共7页Freshwater Fisheries

基　　金：广西创新驱动发展专项(科技重点专项)(桂科AA17204044);中国水产科学研究院黄海水产研究所基本科研业务费(20603022018001)。

摘　　要：实验从山东德州市一罗非鱼(Oreochromis niloticus)养殖场循环水养殖系统的脱氮池中分离到一株具有高效脱氮特性的菌株(编号DZYC02),分别以葡萄糖、蔗糖、可溶性淀粉、丁二酸钠、乙酸钠、柠檬酸钠为碳源,研究了碳源种类对菌株DZYC02脱氨氮效果的影响;同时以蔗糖为碳源、NH4Cl为氮源,研究了不同碳氮比、初始pH及盐度对该菌株脱氮效果的影响。结果显示:菌株DZYC02在以柠檬酸钠为碳源、C∶N≥15、pH 5~7、盐度0~15的条件下具有良好的脱氮效果,24 h内对浓度为20 mg/L的NH+4的去除率达100%,48 h内对浓度为20 mg/L的NO-2去除率高达100%;将该菌采用浸泡方式感染斑马鱼(Danio rerio)进行生物安全试验,结果显示菌株DZYC02对斑马鱼表现出较好的安全特性。分别用Biolog细菌鉴定方法和16S rDNA序列分析比对法对该菌进行鉴定,结果显示菌株DZYC02为一株肺炎克雷伯氏菌(Klebsiella pneumoniae)。A strain of bacteria with denitrification activity was isolated from a tilapia(Oreochromis niloticus)recirculating aquaculture system and designated as DZYC02.Carbon sources including glucose,sucrose,soluble starch,sodium succinate,sodium acetate and sodium citrate were employed to investigate the effects of carbon source on the denitrifying efficacy of strain DZYC02.Also,sodium citrate and ammonia were used as carbon and nitrogen source to evaluate the effects of C∶N,pH and salinity on the efficiency of denitrification.The results showed that the 24 h-denitrifying rate of ammonia(NH+4-N)and 48 h-denitrifying rate of nitrite(NO-2-N)were both 100%under the condition of sodium citrate being used as the carbon source,C∶N≥15,initial pH 5-7 and salinity between 0 and 15.Zebrafish(Danio rerio)were used to evaluate the biosafety of strain DZYC02 by immersion challenge,and the results showed that strain DZYC02 appeared better biosecurity for fish.The strain DZYC02 was identified as Klebsiella pneumoniae by methods of Biolog automated microbes identification system and 16S rDNA sequencing.

关 键 词：罗非鱼(Oreochromis niloticus) 循环水养殖系统 克雷伯氏菌(Klebsiella pneumoniae) 脱氮菌 脱氮效果 

分 类 号：S941.42[农业科学—水产养殖]