食管电极不同置入深度构建心房颤动大鼠模型比较  

Comparison of Rat Models of Atrial Fibrillation with Different Depth of Esophageal Electrode Implantation

作　　者：梁宇明 何燕[1] LIANG Yuming;HE Yan(Department of Cardiology,Gerontology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学第一附属医院老年病学心血管内科,广西南宁530021

出　　处：《心血管病学进展》2020年第4期438-440,F0003,共4页Advances in Cardiovascular Diseases

基　　金：国家自然科学基金(81760061)。

摘　　要：目的比较食管电极不同置入深度致心房颤动(房颤)大鼠模型的诱发率。方法将18只健康成年雄性SD大鼠随机分为3组:心房上部组(A组,n=6)、心房部组(B组,n=6)、心房下部组(C组,n=6)。A、B、C组予不同深度食管电极置入,然后予电刺激起搏左心房诱发房颤发生,重复3次。结果 (1) A、B、C三组的房颤诱发率分别为5.6%、38.9%和27.8%,B、C两组诱发率比A组高,B组与A组差异有统计学意义(P<0. 05),B、C两组和A、C两组组间比较差异无统计学意义(P>0. 05);(2) A、B、C三组食管电极置入深度分别为(6.53±0. 19) cm、(7.15±0. 23) cm和(7.58±0. 75) cm;(3)三组食管心电图P波振幅分别为(1.25±0. 10)m V、(2.10±0. 09) m V和(1.67±0. 12) m V。结论 B组的房颤诱发率最高,通过正态分布法求参考值范围得出,当食管电极置入深度为6. 70~7. 60 cm,此时食管心电图P波振幅最大,参考范围是1.92~2.28 m V,大鼠房颤诱发率最高,因此优先选择该食管电极置入深度参考范围,房颤模型造模成功率最高。Objective To compare the induction rate of rat model of atrial fibrillation( AF) induced by different depth of esophageal electrode implantation. Methods 18 healthy adult male SD rats were randomly divided into three groups: upper atrial group( group A,n =6),atrial group( group B,n = 6) and lower atrial group( Group C,n = 6). In group A,B and C,esophageal electrodes were implanted in different depths,and then pacing of left atrium was performed to induce atrial fibrillation. Results( 1) The induction rates of AF in group A,B and C were 5.6%,38.9% and 27.8% respectively,the induction rate of group B and C were higher than that of group A.The difference between group B and group A was statistically significant( P<0. 05),but there was no significant difference between group B and C,and also between group A and C( P>0. 05).( 2) The insertion depth of esophageal electrodes in group A,B and C were( 6. 53 ± 0. 19) cm,( 7. 15 ±0. 23) cm and( 7.58±0. 75) cm,respectively.( 3) The amplitudes of P wave in three groups were( 1.25±0. 10) m V,( 2.10±0. 09) m V and( 1.67±0. 12) m V respectively. Conclusion Group B has the highest atrial fibrillation induction rate. By using the normal distribution method to calculate the reference range,we can draw the conclusion that when the esophageal electrode implantation depth is 6.70 ~ 7. 60 cm,the esophageal ECG P wave amplitude is the largest,the reference range is 1.92 ~ 2.28 m V,and the rat’s atrial fibrillation induction rate is the highest.Therefore,it’s the first choice of the esophageal electrode implantation depth reference range,which has the highest success rate of atrial fibrillation model.

关 键 词：大鼠模型 食管调搏 心房颤动 

分 类 号：R541.75[医药卫生—心血管疾病] R-332[医药卫生—内科学]