茄子花药开裂相关基因SmDAD1启动子的克隆及功能分析  

Cloning and Functional Analysis of SmDAD1 Promoter in Solanum melongena

作　　者：张少伟 袁超 牛义 汤青林 魏大勇 王永清[2] 田时炳[2] 杨洋[2] 王志敏 ZHANG Shaowei;YUAN Chao;NIU Yi;TANG Qinglin;WEI Dayong;WANG Yongqing;TIAN Shibing;YANG Yang;WANG Zhimin(Chongqing Key Laboratory of Olericulture,Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China;The Institute of Vegetables and Flowers,Chongqing Academy of Agricultural Sciences,Chongqing 400055,China)

机构地区：[1]西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室,重庆400715 [2]重庆市农业科学院蔬菜花卉研究所,重庆400055

出　　处：《园艺学报》2020年第4期643-652,共10页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2016YFD0100204);国家自然科学基金项目(31501756);重庆市自然科学基金项目(cstc2019jcyj-msxm2291);中央高校基本科研业务费专项(XDJK2018B039);重庆市技术创新与应用发展专项(cstc2019jscx-gksbX0149)。

摘　　要：以茄子‘F142’为材料,通过染色体步移法克隆了花药开裂相关基因SmDAD1上游746 bp的启动子序列。通过生物信息在线软件预测,该启动子中含有多个与植物发育及逆境应答相关的顺式作用元件。为进一步分析SmDAD1启动子的功能,构建prSmDAD1::GUS融合表达载体,转化到拟南芥,并对T3代纯合转基因拟南芥进行GUS染色分析。结果表明,SmDAD1启动子在拟南芥幼苗的根部、叶片和花药均有较强活性,其中在根部活性最强,在茎中没有活性。检测转基因植株GUS基因的表达量发现,SmDAD1启动子受茉莉酸甲酯(MeJA)、脱落酸(ABA)、水杨酸(SA)、赤霉素(GA)、生长素(IAA)和1-氨基环丙烷-1-羧酸(ACC)诱导。因此推测SmDAD1可能在茄子发育和抗外界逆境胁迫中起重要作用。The promoter of SmDAD1 gene with 746 bp was cloned from eggplant accession‘F142’.Bioinformatics online software prediction showed that the promoter contained a plurality of cis-acting elements related to plant development and stress response.In order to further analyze the function of SmDAD1 promoter,prSmDAD1::GUS fusion expression vector was constructed.It was transferred into Arabidopsis thaliana,and GUS staining analysis was performed on T3 generation homozygous transgenic Zhang Shaowei,Yuan Chao,Niu Yi,Tang Qinglin,Wei Dayong,Wang Yongqing,Tian Shibing,Yang Yang,Wang Zhimin.Cloning and functional analysis of SmDAD1 promoter in Solanum melongena.644 Acta Horticulturae Sinica,2020,47(4):643-652.plant.The results showed that SmDAD1 promoter had very high activity in the roots,leaves and anthers,especially in the roots,but no activity in stems.Detection of GUS gene expression in transgenic plants indicated that SmDAD1 promoter was induced by MeJA,ABA,SA,GA,IAA and ACC.Therefore,we speculate that SmDAD1 may play important role in eggplant development and resistance to external stress.

关 键 词：茄子 启动子 SmDAD1 GUS基因 功能分析 

分 类 号：S641.1[农业科学—蔬菜学]