小半夏汤类方防治延迟性化疗性呕吐的药效及机制研究  

Efficacy and mechanism of Xiaobanxia Decoction and categorized formula in preventing and treating delayed chemotherapeutic vomiting

作　　者：赵吉艳 李金曦 张义敏 董振飞 王均宁 Zhao Jiyan;Li Jinxi;Zhang Yimin;Dong Zhenfei;Wang Junning(College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学中医学院,济南250355

出　　处：《国际中医中药杂志》2020年第2期138-143,共6页International Journal of Traditional Chinese Medicine

基　　金：山东省重点研发计划(产业关键技术)项目(2016CYJS08A01-3、2016CYJS08A01-4);山东省重点研发计划(2016ZDJS07A12);山东中医药大学科学基金(2018zk06)。

摘　　要：目的观察小半夏汤类方对顺铂诱导的大鼠延迟性化疗性呕吐的药效,探讨其作用机制。方法将60只Wistar大鼠按随机数字表法分为空白组、模型组、昂丹司琼组、小半夏汤组、生姜半夏汤组和半夏干姜散组,每组10只。昂丹司琼组灌胃盐酸昂丹司琼注射液2.6 mg/(kg·d),小半夏汤组灌胃小半夏汤水煎液22 g/(kg·d),生姜半夏汤组灌胃生姜半夏汤水煎液31.5 ml/(kg·d),半夏干姜散组灌胃半夏干姜散水煎液0.63 g/(kg·d),空白组和模型组灌胃等体积生理盐水。每天灌胃2次,连续给药3 d。首次给药后1 h,除空白组外,其余各组大鼠腹腔注射6 mg/kg顺铂建立化疗性呕吐模型。每24 h称量大鼠高岭土摄入量,采用ELISA法检测大鼠回肠和延髓5-羟色胺(5-hydroxytryptamine,5-HT)、5-羟色胺吲哚乙酸(pentahydroxytryptamine indoleacetic acid,5-HIAA)、色氨酸羟化酶(trptophan hydroxylase,TPH)、单胺氧化酶A(monoamine oxidases A,MAOA)水平。结果与模型组比较,小半夏汤组、生姜半夏汤组、半夏干姜散组大鼠回肠和延髓5-HT[回肠(308.04±29.90)ng/L、(355.97±19.16)ng/L、(389.97±24.86)ng/L比(498.95±13.92)ng/L,延髓(375.32±19.33)ng/L、(395.18±16.12)ng/L、(406.68±12.09)ng/L比(478.52±13.88)ng/L]水平均降低(P<0.05),小半夏汤组回肠TPH[(35.14±2.68)ng/L比(47.31±0.83)ng/L]水平降低(P<0.05),生姜半夏汤组、半夏干姜散组延髓TPH[(33.68±2.79)ng/L、(38.19±1.74)ng/L比(43.68±1.53)ng/L]水平降低(P<0.05),小半夏汤组、生姜半夏汤组、半夏干姜散组大鼠回肠和延髓MAOA、5-HIAA水平均降低(P<0.05)。结论小半夏汤类方可有效防治大鼠延迟性化疗性呕吐,其机制可能与降低TPH水平、抑制5-HT合成有关。Objective To observe the pharmacodynamic effect of Xiaobanxia Decoction(XBXD)and categorized formula on cisplatin-induced delayed chemotherapeutic vomiting in rats,and to explore its prevention and treatment mechanism.Methods Sixty Wistar rats were randomly divided into blank group,model group,ondansetron group,XBXD group,Ginger Pinellia Decoction group and Pinellia and Dried Ginger Powder group,with 10 rats in each group.The ondansetron group was given 2.6 mg/(kg•d)ondansetron,the XBXD group was given XBXD 22 g/(kg•d),the Ginger Pinellia Decoction group was given 31.5 ml/(kg•d)Ginger Pinellia Decoction,and Pinellia and Dried Ginger Powder group was given 0.63 g/(kg•d)Dried Ginger Powder.The blank group and the model group were intragastrically administered with normal saline.All the treattmens were last for three days and twice per day.After the first administration,except the blank group,the rats in all groups were intraperitoneally injected with 6 mg/kg cisplatin to establish chemotherapy-induced vomiting model.The kaolin intake of the chemotherapical rats were weighed every 24 h,and the ileum and medulla 5-HT,5-HIAA,TPH and MAOA levels were detected by ELISA.Results Compared with the model group,the kaolin intake of the chemotherapy rats in each treatment group significantly decreased(P<0.05).The levels of ileum 5-HT(308.04±29.90 ng/L,355.97±19.16 ng/L,389.97±24.86 ng/L vs.498.95±13.92 ng/L)and medulla 5-HT(375.32±19.33 ng/L,395.18±16.12 ng/L,406.68±12.09 ng/L vs.478.52±13.88 ng/L)in the XBXD group,Ginger Pinellia Decoction group and Pinellia and Dried Ginger Powder significantly decreased(P<0.05).The levels of ileum TPH(35.14±2.68 ng/L vs.47.31±0.83 ng/L)in the XBXD group significantly decreased(P<0.05).The levels of medulla TPH(33.68±2.79 ng/L,38.19±1.74 ng/L vs.43.68±1.53 ng/L)in the Ginger Pinellia Decoction group and Pinellia and Dried Ginger Powder significantly decreased(P<0.05).The levels of MAOA,5-HIAA in the ileum and medulla of the XBXD group,Ginger Pinellia Decoction group and

关 键 词：小半夏汤 延迟性化疗性呕吐 5-羟色胺 色氨酸羟化酶 大鼠 

分 类 号：R28[医药卫生—中药学]