基于网络药理学的当归-白芍药对治疗心血管疾病作用机制研究  

Study on the mechanism of two related Chinese herbs Danggui-Baishao in treating cardiovascular diseases based on network pharmacology

作　　者：江晓泉 刘洋[1] 李雪岩 韩星 毛斌[2] Jiang Xiaoquan;Liu Yang;Li Xueyan;Han Xing;Mao Bin(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Department of Cardiac Surgery,Capital Medical College Affiliated Beijing Anzhen Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学中药学院,102488 [2]首都医科大学附属北京安贞医院心脏外科,100029

出　　处：《国际中医中药杂志》2020年第2期151-156,共6页International Journal of Traditional Chinese Medicine

摘　　要：目的利用网络药理学技术研究经典药对当归-白芍治疗心血管疾病的作用及机制。方法检索TCMSP和相关文献筛选得到当归-白芍药对中潜在活性化合物,并结合SWISS、STITCH数据库挖掘化合物对应的靶点;通过治疗靶标数据库(TTD)、在线人类孟德尔遗传数据库(OMIM)和DrugBank数据库挖掘心血管疾病靶点;构建蛋白互作网络,分析主要靶点;利用生物学信息注释数据库(DAVID 6.8)进行KEGG信号通路注释分析,并应用Cytoscape构建成分-主要靶点-关键通路网络。结果构建的成分-靶点-通路网络中包括17个药对成分,54个主要靶点及10条关键通路。涉及的通路包括炎症相关通路(TNF信号通路)、心血管系统相关通路(PI3K-AKT信号通路、FoxO信号通路、VEGF信号通路、Rap1信号通路等)、催乳素信号通路和雌激素信号通路。结论应用网络药理学验证了当归-白芍药对多成分、多靶点、整体调节的作用特点,推测其主要通过调节血管生成、炎症反应和细胞凋亡等途径发挥治疗心血管疾病的作用。Objective By adopting network pharmacology to study the mechanism of the two classical Chinese herbs Danggui-Baishao in treating cardiovascular diseases.Methods By searching from TCMSP and related literature,together with the databases of TCMSP,SWISS and STITCH,potential active compounds of Danggui-Baishao were collected,while the targets for cardiovascular diseases were obtained from TTD,OMIM and DrugBank databases.Then the PPI network was screened for the major targets.The KEGG Pathway annotation analyses of major targets were performed by using the DAVID database.The ingredient-major target-key pathway network was constructed by Cytoscape.Results There were 17 compounds and 54 major targets in the ingredient-target-pathway network,as well as 10 key pathways,including inflammation-related pathway(TNF signaling pathway),pathways related to cardiovascular system(such as PI3K-Akt signaling pathway,FoxO signaling pathway,VEGF signaling pathway,Rap1 signaling pathway),prolactin signaling pathway and estrogen signaling pathway.Conclusions The study verified the characteristics of multi-components,multi-targets and integral regulation for Danggui-Baishao with the application of network pharmacology.It predicted that Dangui-Baishao couldtreat cardiovascular diseases mainly by regulating angiogenesis,inflammatory response and apoptosis.

关 键 词：当归 白芍 药对 心血管疾病 网络药理学 

分 类 号：R28[医药卫生—中药学]