积雪草-大黄不同配伍比例对积雪草有效成分溶出率的影响  

Effect of different compatibility ratios of Centella asiatica-Rhubarb on the dissolution rate of effective components of Centella asiatica

作　　者：韩怡[1] 柳春娣 余辉 李杰[1] Han Yi;Liu Chundi;Yu Hui;Li Jie(Pharmacy Department of Zhangjiagang Hospital Affiliated to Nanjing University of Traditional Chinese Medicine,Zhangjiagang 215600,China)

机构地区：[1]南京中医药大学附属张家港医院药学部,215600

出　　处：《国际中医中药杂志》2020年第1期58-61,共4页International Journal of Traditional Chinese Medicine

基　　金：苏州市科技发展计划-产业技术创新专项(民生科技-医疗卫生应用基础研究)项目(SYSD2016161)。

摘　　要：目的:研究积雪草-大黄不同配伍比例对积雪草有效成分羟基积雪草苷和积雪草苷溶出率的影响。方法:采用HPLC法测定积雪草单提液及积雪草-大黄6种配伍比例混合提取液中羟基积雪草苷和积雪草苷含量。色谱条件:Thermo C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-2 mmol/L的β-环糊精溶液,流速1 ml/min,柱温30℃,检测波长205 nm。结果:与积雪草单提液比较,积雪草-大黄配伍对羟基积雪草苷含量影响较大,羟基积雪草苷溶出率随大黄比例增大而降低。积雪草-大黄配伍对积雪草苷含量影响较小。结论:综合考虑积雪草的临床应用及功效,积雪草-大黄较佳配比为10∶1及15∶1。Objective To study the effects of different compatibility ratios of Centella asiatica-Rhubarb on the dissolution rates of madecassoside and asiaticoside.Methods By using the HPLC method to analyze the content of madecassoside and asiaticoside in the Centella asiatica extract solution as well as Centella asiatica-Rhubarb extract solution with 6 different kinds of compatibility ratios.The tests were carried out by Thermo C18(250 mm×4.6 mm,5μm)by isocratic elution with acetonitrile and 2 mmol/L of beta-cyclodextrin solution as mobile phase at a flow rate of 1 ml/min,the column temperature was 30℃and the detection wavelength was 205 nm.Results Compared with the Centella asiatica extract solution,the dissolution rate of madecassoside decreased significantly with the increase of the proportion of rhubarb.The combination of Centella asiatica and Rhubarb had little effect on the content of asiaticoside.Conclusions Considering the clinical application and efficacy of Centella asiatica,the optimal ratio of Centella asiatica-Rhubarb is 10:1 and 15:1.

关 键 词：积雪草 大黄 中药配伍 羟基积雪草苷 积雪草苷 含量测定(中药) 

分 类 号：R284[医药卫生—中药学]