解毒止血汤水提液中黄芩苷、汉黄芩苷、丹皮酚含量及其理化参数测定  

The parametric measurementof physico-chemical parameter and three types of effective components in Jiedu-Zhixue Decoction

作　　者：刘李梅 谢松 彭伟 向猛[1] 郭宏彦[1] 涂禾[1] Liu Limei;Xie Song;Peng Wei;Xiang Meng;Guo Hongyan;Tu He(Department of Pharmaceutical Preparation,Sichuan Provincial Orthopedic Hospital,Chengdu 610041,China)

机构地区：[1]四川省骨科医院制剂室,成都610041

出　　处：《国际中医中药杂志》2020年第1期62-66,共5页International Journal of Traditional Chinese Medicine

基　　金：四川省骨科医院科研项目(2017-9)。

摘　　要：目的:建立测定解毒止血汤水提液中黄芩苷、汉黄芩苷、丹皮酚含量的方法,并测定其理化参数。方法:采用HPLC法检测解毒止血汤水提液中黄芩苷、汉黄芩苷、丹皮酚含量。色谱柱为Waters Symmetry Shield TMRP18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速为1 ml/min,检测波长274 nm,柱温30℃。测定解毒止血汤水提液的pH值及相对密度。结果:黄芩苷在0.1912~1.1472μg范围内线性良好,汉黄芩苷在0.0415~0.2074μg范围内线性良好,丹皮酚在0.0195~0.1563μg范围内线性良好。该方法重现性、稳定性、回收率均良好。3批解毒止血汤水提液中黄芩苷、汉黄芩苷、丹皮酚含量无显著差异,其pH值范围为5.240~5.753,相对密度范围为0.846~0.889。结论:本文所建立方法可测定解毒止血汤水提液中多个有效成分,方法稳定、可行,适用于解毒止血汤水提液的质量控制。Objective To set up a method to measure the content of baicaline,wogonoside,and paeonol in Jiedu-Zhixue Decoction extract and to measure their physico-chemical parameters.Methods By using HPLC analytical methods to measure the content of baicaline,wogonoside,and paeonol in Jiedu-Zhixue Decoction extract.The RP18 column was adopted,gradient eluted with mobile phase of acetonitrile-1%phosphoric acid water,flow rate was 1 ml/min,detection wavelength was 274 nm,and the column temperature was 30℃.The method quantitatively analyze the compound extracts of baicaline,wogonoside and paeonol were used.Results The method isrepeatable,stable with good recorey rates.The calibration curves of baicaline was in good linearity over the range of 0.1912-1.1472μg.The calibration curves of wogonoside was in good linearity over the range of 0.0415-0.2074μg.The calibration curves of paeonol was in good linearity over the range of 0.0195-0.1563μg.There was no significant difference among the component of 3 different batches of Jiedu-Zhixue Decoction extract,and the pH value range was 5.240-5.753,relative density range was 0.846-0.889.Conclusions The method used in this stydy to measure the effective components of Jiedu-Zhixue Decoction extract was stable and feasible,which is suitable for quality control of Jiedu-Zhixue Decoction.

关 键 词：剂型改进 解毒止血汤 黄芩苷 汉黄芩苷 丹皮酚 理化参数 高效液相色谱法 质量控制 

分 类 号：R28[医药卫生—中药学]