圣草次苷-CTAB-DNA三元体系共振光散射光谱研究及应用  

Resonance light scattering spectroscopy of eriocitrin-CTAB-DNA ternary and its application

作　　者：邵谛 赵瑞 孙晓悦 吴鋆 毕淑云 SHAO Di;ZHAO Rui;SUN Xiao-yue;WU Jun;BI Shu-yun(College of Chemistry,Changchun Normal University,Changchun 130032,China)

机构地区：[1]长春师范大学化学学院,吉林长春130032

出　　处：《分子科学学报》2020年第1期56-61,I0004,共7页Journal of Molecular Science

基　　金：吉林省科技厅资助项目(20170101020JC).

摘　　要：基于圣草次苷和十六烷基三甲基溴化胺(CTAB)的二元体系增强DNA的共振光散射(resonance light scattering,RLS)信号,提出了一种测定纳克级小牛胸腺DNA(ctDNA)的新方法.研究了圣草次苷、CTAB与DNA的结合方式并讨论了三元体系的最佳反应条件.增强的RLS信号与DNA浓度具有良好的线性关系,线性方程为ΔIRLS=28.33+1241c,r=0.9953.采用该方法对合成样品进行了分析测定,回收率和RSD分别为96.51%~101.38%和2.56%~3.90%,检出限为1.942μg·L^-1.The binary system based on eriocitrin and CTAB enhanced the resonance light scattering of DNA.We proposed a new method for the determination of nanogram-scale ctDNA,and explored the optimal reaction conditions for the ternary system.The synthetic sample was analyzed and determined according to the method.Enhanced RLS signal has a good linear relationship with the concentration of DNA.The lineer equation wasΔIRLS=28.33+1241c,r=0.9953.The recoveries and RSD were 96.51%~101.38%and 2.56%~3.90%,respectively,and the detection limit was 1.942μg·L^-1.

关 键 词：共振光散射 小牛胸腺DNA 圣草次苷 CTAB 

分 类 号：O657[理学—分析化学]