Better understanding of the activated sludge process combining fluorescence-based methods and flow cytometry:A case study  

作　　者：Vanesa Benito Javier Etxebarria Felipe Goni-de-Cerio Inigo Gonzalez Pilar Brettes Ana Urkiaga 

机构地区：[1]GAIKER,Parque Tecnológico,Ed.202,48170,Zamudio,Bizkaia,Spain [2]EDAR Galindo,Bilbao Bizkaia Water Consortium,Sestao 48910,Bizfcaia,Spain

出　　处：《Journal of Environmental Sciences》2020年第4期51-58,共8页环境科学学报（英文版）

基　　金：supported by the Bilbao Bizkaia Water Consortium

摘　　要：This study aims to demonstrate the validity of fluorescence-based methods,together with flow cytometry,as a complementary tool to conventional physicochemical analyses carried out in wastewater treatment plants(WWTPs),for the control of the currently largely unknown activated sludge process.Staining with SYTO 9,propidium iodide and 5-(and 6)-carboxy-2’,7’-difluorodihydrofluorescein diacetate(carboxy-H2 DFFDA)was used for cell viability and oxidative stress monitoring of the bacterial population forming the activated sludge of a WWTP.Throughout the period of research,several unstable periods were detected,where the non-viable bacteria exceeded the 75%of the total bacterial population in the activated sludge,but only in one case the cells with oxidative stress grew to 9%,exceeding the typical values of2%-5%of this plant.These periods coincided in two cases with high values of total suspended solids(SST)and chemical oxygen demand(COD)in the effluent,and with an excess of ammonia in other case.A correlation between flow cytometric and physicochemical data was found,which enabled to clarify the possible origin of each case of instability in the biological system.This experience supports the application of bacterial fluorescence staining,together with flow cytometric analysis,as a simple,rapid and reliable tool for the control and better understanding of the bacteria dynamics in a biological wastewater treatment process.

关 键 词：Activated sludge Wastewater treatment Cell viability Oxidative stress Fluorescent dyes Flow cytometry 

分 类 号：X703[环境科学与工程—环境工程]