Verification of Bacteroidales 16S rRNA markers as a complementary tool for detecting swine fecal pollution in the Yangtze Delta  

作　　者：Lihua Fan Xiaofeng Zhang Ruoxue Zeng Suhua Wang Chenchen Jin Yongqiang He Jiangbing Shuai 

机构地区：[1]Department of Food Science and Nutrition,Zhejiang Key Laboratory for Agro-Food Processing,Zhejiang University,Hangzhou 310016,China [2]Zhejiang Academy of Science and Technology for Inspection and Quarantine,Hangzhou 310058,China

出　　处：《Journal of Environmental Sciences》2020年第4期59-66,共8页环境科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2016YFD0501105);the Science and Technology Department of Zhejiang Province(No.2015C02044);the National Natural Science Foundation of China(No.31301492)

摘　　要：To correctly assess and properly manage the public health risks associated with exposure to contaminated water,it is necessary to identify the source of fecal pollution in a watershed.In this study,we evaluated the efficacy of our two previously developed real time-quantitative PCR(qPCR)assays for the detection of swine-associated Bacteroidales genetic markers(gene 1-38,gene 3-53)in the Yangtze Delta watershed of southeastern China.The results indicated that the gene 1-38 and 3-53 markers exhibited high accuracy(92.5%,91.7%conditional probability,respectively)in detecting Bacteroidales spp.in water samples.According to binary logistic regression(BLR),these two swine-associated markers were well correlated(P<0.05)with fecal indicators(Escherichia coli and Enterococci spp.)and zoonotic pathogens(E.coli O157:H7,Salmonella spp.and Campylobacter spp.)in water samples.In contrast,concentrations of conventional fecal indicator bacteria(FIB)were not correlated with zoonotic pathogens,suggesting that they are noneffective at detecting fecal pollution events.Collectively,the results obtained in this study demonstrated that a swinetargeted qPCR assay based on two Bacteroidales genes markers(gene 1-38,gene 3-53)could be a useful tool in determining the swine-associated impacts of fecal contamination in a watershed.

关 键 词：Bacteroidales genes markers Water pollution Real-time quantitative PCR Fecal indicators Zoonotic pathogens 

分 类 号：X713[环境科学与工程—环境工程]