白芍对酒精性肝损伤肝阴虚证的保护功效和机制  

Effects of Baishao on liver yin deficiency pattern in alcoholic liver injury and its mechanism

作　　者：贾岚 王蕾蕾 孟靓 华姞安 付璐 姚骏凯 高学敏 王景霞 Jia Lan;Wang Leilei;Meng Liang;Hua Ji’an;Fu Lu;Yao Junkai;Gao Xuemin;Wang Jingxia(School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中医学院,北京100029

出　　处：《北京中医药大学学报》2020年第3期203-211,共9页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81673617);国家中医药管理局中医药行业科研专项(No.201507004)。

摘　　要：目的研究白芍对小鼠酒精性肝损伤肝阴虚证的保护功效和机制。方法将ICR小鼠随机分为空白组、模型组、一贯煎组、白芍组,每组10只,除空白组外,用Lieber-DeCarli酒精液体饲料加甲状腺激素灌胃制备小鼠酒精性肝损伤肝阴虚证模型。造模期第1天开始给药,一贯煎组灌胃一贯煎水提液9.07 g/kg,白芍组灌胃白芍水提液4.3 g/kg,空白组和模型组灌胃等量生理盐水。28 d后观察各组小鼠状态,测定体重、体温、心率,测定血清及肝组织转氨酶活性、细胞因子水平、乙醇及脂肪代谢相关酶活性、抗氧化酶活性并取肝脏进行冰冻油红、HE病理切片检查。结果与空白组相比,模型组小鼠体重明显减轻,体温升高,心率加快,且出现毛发无光泽、疲倦易困、烦躁易怒等症状;血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)、总胆固醇(TC)、环磷酸腺苷(cAMP)水平及肝组织丙二醛(MDA)、细胞色素P450同工酶E1(CYP2E1)、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)水平明显升高;血清中环磷酸鸟苷(cGMP)水平及肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、乙醇脱氢酶(ADH)、乙醛脱氢酶2(ALDH2)、白细胞介素10(IL-10)、白细胞介素1Ra(IL-1Ra)水平明显降低;肝脏出现脂质沉积及肝细胞坏死情况。与模型组相比,白芍组小鼠血清中ALT、AST、TG、TC、cAMP水平均明显降低(P<0.05),cGMP水平明显升高(P<0.05);肝组织中CYP2E1、TNF-α、IL-1β、IL-6水平明显降低(P<0.05),SOD、CAT、ADH、ALDH2、IL-10、IL-1Ra水平明显升高(P<0.05);肝组织脂质占比显著降低(P<0.05),肝细胞坏死程度改善。结论白芍具有明显的保护酒精性肝损伤肝阴虚证的作用,其机制与调节cAMP/cGMP水平及细胞因子水平、使肝细胞功能与代谢恢复平衡、增强肝组织中乙醇代谢关键酶及抗氧化酶活性、同时抑制脂质过氧化反应、减少脂质沉积有关。Objective To study the effects of Baishao(White Peony Root, Radix Paeoniae Alba) on liver yin deficiency pattern in mice with alcoholic liver injury and mechanism. Methods ICR mice were randomly divided into blank group, model group, Yiguan Jian(Effective Integration Decoction, YGJ) group and Baishao group, with 10 mice in each group. Mice models of alcoholic liver injury with liver yin deficiency pattern were established by intragastric administration of Lieber-DeCarli alcohol diet plus thyroid hormone. Meanwhile, the blank group and the model group were given physiological saline by gavage, the YGJ group was given YGJ(9.07 g/kg, 20 mL/kg) by gavage, and the Baishao group baishao exact(4.3 g/kg, 20 mL/kg) by gavage. After 28 days, the state of mice in each group was observed, and their body weight, body temperature and heart rate were measured. Levels of transaminase activities, cytokine, enzyme activities related to ethanol and fat metabolism and antioxidant enzyme activities of the serum and liver tissue were detected. Frozen liver sections were examined with Oil Red O staining and liver biopsies with H&E staining. Results Compared with the blank group, the weight of the mice in the model group was significantly reduced, the body temperature increased, the heart rate increased, accompanied with symptoms like lusterless hair, tiredness, sleepiness and irritability. The levels of serum ALT, AST, TG, TC and cAMP and levels of liver MDA, CYP2 E1, TNF-α, IL-1β and IL-6 in the model group were significantly increased. The serum levels of cGMP and liver tissue levels of SOD, CAT, ADH, ALDH2, IL-10 and IL-1 Ra were significantly reduced. In addition, lipid deposition and hepatocyte necrosis occurred in the liver. Compared with the model group, serum levels of ALT, AST, TG, TC and cAMP in the Baishao group were significantly lowered(P<0.05), and cGMP levels were significantly increased(P<0.05). The liver levels of CYP2 E1, TNF-α, IL-1β and IL-6 were significantly decreased(P<0.05), and the levels of SOD, CAT, ADH,

关 键 词：白芍 酒精性肝损伤 肝阴虚证 细胞因子 小鼠 

分 类 号：R285.5[医药卫生—中药学]