白虎汤相态拆分及不同相态中主要成分的含量测定  

Determination of Main Components in Baihutang with Separation of Different Phase States and Different Phase States

作　　者：吕邵娃 武印奇 李英鹏 王艳宏 杨志欣 王锐 管庆霞 李永吉 LYU Shao-wa;WU Yin-qi;LI Ying-peng;WANG Yang-hong;YANG Zhi-xin;WANG Rui;GUAN Qing-xia;LI Yong-ji(Key Laboratory of Basic Research on Pharmacodynamics Substances of Traditional Chinese Medicine(TCM)and Natural Medicine in Heilongjiang Province,Heilongjiang University of Chinese Medicine,Harbin 150040,China;School of Chinese Material Medica,Tianjin University of TCM,Tianjin 300193,China)

机构地区：[1]黑龙江中医药大学黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨150040 [2]天津中医药大学中药学院,天津300193

出　　处：《中国实验方剂学杂志》2020年第11期154-160,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81673611)。

摘　　要：目的:对白虎汤药液进行相态拆分,并对白虎汤真溶液相态、纳米相态及沉淀相态中的芒果苷、新芒果苷、钙离子、甘草苷和甘草酸铵的含量进行测量,以明确白虎汤纳米粒子对其主要成分的增溶作用,从而阐释白虎汤强效解毒解热作用机制。方法:采用高速离心和透析技术对白虎汤进行相态拆分,并采用高效液相色谱法对白虎汤不同相态中的芒果苷、新芒果苷、甘草苷和甘草酸铵的含量进行测定。采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-25 mmol·L^-1磷酸二氢钾溶液梯度洗脱,检测波长257 nm,柱温30℃,流速1.0 mL·min^-1。采用乙二胺四乙酸二钠(EDTA-2Na)溶液对白虎汤不同相态中钙离子浓度进行标定。结果:1 mL白虎汤纳米粒子中含有新芒果苷483.00μg,芒果苷1068.88μg,甘草苷219.93μg,甘草酸铵187.10μg,其中新芒果苷和芒果苷的含量分别占1 mL白虎汤的89.4%和89.9%。纳米相态中新芒果苷和芒果苷分别是真溶液230.0倍和23.3倍,沉淀的8.5倍和14.4倍。白虎汤纳米相态中含有钙离子的含量较多,占白虎汤水煎液的86.9%,且白虎汤及白虎汤纳米相态中钙离子含量均高于石膏组。结论:白虎汤纳米相态的主要成分含量明显高于其他相态,白虎汤纳米粒子对于白虎汤中主要解热成分新芒果苷、芒果苷和钙离子以及解毒成分甘草酸和甘草次酸起到了增溶作用,白虎汤强效解毒解热作用机制与其形成的纳米粒子有关。Objective:The phase separation of Baihutang was carried out.The content of mangiferin,new mangiferin,calciumion,glycyrrhizin and ammonium glycyrrhizinate in the solution phase,the nano phase and the precipitated phase of Baihutang were measured,so as to define the effect of nanometer particles of Baihutang on the growth of active components,and explain the mechanisms of Baihutang in potent detoxification and heat removal.Method:The phase separation of Baihutang was performed by high-speed centrifugation and dialysis.The contents of mangiferin,new mangiferin,glycyrrhizin and ammonium glycyrrhizinate in Baihutang were determined by HPLC.Chromatographic column Diamonsil C18(4.6 mm×250 mm,5μm)was adopted,with acetonitrile-25 mmol·L^-1 potassium dihydrogen phosphate solution as the mobile phase,and eluted in a gradient mode.The detection wavelength was 257 nm,the column temperature was 30℃,and the flow rate was 1.0 mL·min^-1.EDTA-2Na solution was used to calibrate the calcium concentration in different phase states of Baihutang.Result:1 mL Baihutang nanoparticles contained 483.00μg new mangiferin,1068.88μg mangiferin,219.93μg glycyrrhizin and 187.10μg ammonium glycyrrhizin,and the content of new mangiferin and mangiferin accounted for 89.4%and 89.9%respectively in 1 mL Baihutang.The new mangiferin and mangiferin in the nano phase were 230.0 and 23.3 times the true solution,and 8.5 and 14.4 times of the precipitation,respectively.The content of calcium ions in Baihutang in the nano phase was higher,accounting for 86.9%of Baihutang,and the content of calcium ions in Baihutang and Baihutang in the nano phase was higher than that in gypsum group.Conclusion:The content of main components in Baihutang in nanometer phase is significantly higher than that in other phases.The nanoparticles of Baihutang have a solubilizing effect on the main antipyretic components,such as mangiferin,mangiferin and calcium ions as well as the antitoxic components glycyrrhizic acid and glycyrrhizic acid.The mechanism of action of Baihut

关 键 词：白虎汤 相态 纳米粒子 含量测定 

分 类 号：R284.1[医药卫生—中药学] R289[医药卫生—中医学]