厚朴七物汤物质基准特征图谱的建立  

Establishment of Material Benchmark Characteristic Spectrum of Houpu Qiwutang

作　　者：孙娜 吴佳妮 孔慧[1] 李艳英 屈会化 SUN Na;WU Jia-ni;KONG Hui;LI Yan-ying;QU Hui-hua(School of Chinese Materia Medica,School of Traditional Chinese Medicine(TCM),Beijing Institute of TCM,Beijing University of Chinese Medicine,Beijing 100029,China;Beijing Increase Innovation Pharmaceutical Co.Ltd.,Beijing 102200,China)

机构地区：[1]北京中医药大学中药学院,中医学院,北京中医药研究院,北京100029 [2]北京盈科瑞创新药物研究有限公司,北京102200

出　　处：《中国实验方剂学杂志》2020年第11期178-184,共7页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立厚朴七物汤物质基准的特征图谱。方法:针对方中各药味的功效与药理作用,筛选特征图谱目标物质,针对各药味关联的药效成分进行提取溶剂、检测波长、洗脱梯度优化,将单味药与其对比,进行峰归属。采用Q-TOF对特征峰进行分子式的推断,同时利用对照品进行特征峰的确认,筛选出参照物。结果:建立的厚朴七物汤物质基准特征图谱中,7个药味中5个药味得到归属。对9个特征峰进行质谱解析,其中7个特征峰与对照品确认,分别为甘草苷、柚皮苷、新橙皮苷、甘草酸铵、大黄酸、和厚朴酚、厚朴酚,选取新橙皮苷作为参照物,并根据特征峰的分离情况及保留时间,确定以新橙皮苷峰作为特征图谱的S峰。测定10批厚朴七物汤物质基准特征图谱,生成对照特征图谱,确定各特征峰的相对保留时间。结论:通过对特征峰的质谱解析以及对重点成分进行把控,建立了厚朴七物汤物质基准的特征图谱。不仅实现了对该方全部药效成分的有效控制,还实现了对该方重点功效成分的把控,为后续厚朴七物汤制剂的开发及质量控制提供理论依据。Objective:To establish a characteristic spectrum to reflect the efficacy of Houpo Qiwutang.Method:Based on the correlation between the efficacy and the pharmacological action of each herb in prescription,the target substances of characteristic map were screened.The extraction solvent,detection wavelength and gradient of the active ingredients were optimized.Peak assignment was made by comparing individual drugs.Q-TOF was used to infer the molecular formula of each peak in the characteristic atlas,and the reference substance was identified by the reference substance.The reference substance was screened out according to the correlation of main efficacy and medicine.Result:The characteristic spectrum of material standard of Houpu Qiwutang was established.Five of the seven herbal medicines were attributed.Nine characteristic peaks were selected and identified by Q-TOF as glycyrrhizin,including naringin,neohesperidin,ammonium glycyrrhizinate,rhein,honokiol,magnolol.According to the main efficacy of Houpo Qiwutang,neohesperidin was selected as reference substances.According to the separation of characteristic peaks and the retention time,the mark peak of the characteristic spectrum was determined.Conclusion:The characteristic spectrum of the material basis of Houpo Qiwutang was established by selecting the characteristic peaks andcontrolling the key components. This method not only reflects the situation of all the effective chemicalcomponents,but also focuses on the control of the key efficacy,so as to provide a theoretical basis for thesubsequent development and quality control of Houpu Qiwu Tang.

关 键 词：厚朴七物汤 物质基准 药理作用 特征图谱 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学]