养精种玉汤对小鼠卵母细胞体外成熟及PDGFA和PDGFRα表达的影响  

Effect of Yangjing-Zhongyu decoction on in vitro maturation and expression of PDGFA and PDGFRα of mouse oocytes

作　　者：肖彩仙 段恒[1] 唐立明 XIAO Cai-xian;DUAN Heng;TANG Li-ming(College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学中医药学院,重庆400016

出　　处：《中国病理生理杂志》2020年第5期918-923,共6页Chinese Journal of Pathophysiology

基　　金：重庆市卫生和计生委中医药科技项目(No.ZY20150251)。

摘　　要：目的:探讨养精种玉汤对小鼠卵母细胞体外成熟及血小板源性生长因子A亚基(platelet-derived growth factor subunit A,PDGFA)和血小板源性生长因子受体α(platelet-derived growth factor receptorα,PDGFRα)表达的影响。方法:SPF级雌性KM小鼠灌胃养精种玉汤后取血制备血清,养精种玉汤血清用于未成熟卵母细胞-颗粒细胞复合体培养。体外培养后,观察并计算小鼠卵母细胞成熟率、受精率和卵裂率,采用Western blot和real-time PCR分别检测卵母细胞PDGFA和PDGFRα的蛋白及mRNA表达情况。结果:养精种玉汤能明显提高卵母细胞体外成熟率和受精率(P<0.05或P<0.01),下调PDGFA和PDGFRα的蛋白和mRNA表达(P<0.01)。结论:养精种玉汤可能通过下调PDGFA和PDGFRα的表达促进小鼠卵母细胞体外成熟。AIM:To investigate the effects of Yangjing-Zhongyu decoction on the in vitro maturation and expression of platelet-derived growth factor subunit A(PDGFA)and platelet-derived growth factor receptor α(PDGFRα)of mouse oocytes.METHODS:The SPF female KM mice were given Yangjing-Zhongyu decoction,and the blood was collected to prepare serum.The serum containing Yangjing-Zhongyu decoction was used to culture immature oocyte-granulosa cell complexes.After the in vitro culture,the oocyte maturation rate,fertilization rate and cleavage rate were observed and calculated,and the expression of PDGFA and PDGFRα in the oocytes at protein and mRNA levels was determined by Western blot and real-time PCR.RESULTS:Yangjing-Zhongyu decoction increased the in vitro maturation rate and fertilization rate of the oocytes(P<0.05 or P<0.01),and down-regulated the protein and mRNA expression of PDGFA and PDGFRα(P<0.01).CONCLUSION:Yangjing-Zhongyu decoction may promote the in vitro maturation of mouse oocytes by down-regulating the expression of PDGFA and PDGFRα.

关 键 词：养精种玉汤 卵母细胞 血小板源性生长因子A亚基 血小板源性生长因子受体α 

分 类 号：R285.2[医药卫生—中药学] R363.2[医药卫生—中医学]