EGCG及其络合物EGCG-Cu的消毒效果研究  

Disinfection effect of EGCG and EGCG-Cu complexes

作　　者：庆杉 赵岩 冯萃敏[1,2] 段鹏鑫[4] 王婷[1,2] 张欣蕊 丁梓沁 QING Shan;ZHAO Yan;FENG Cui-min;DUAN Peng-xin;WANG Ting;ZHANG Xin-rui;DING Zi-qin(Beijing Advanced Innovation Center for Future Urban Design,Beijing University of Civil Engineering and Architecture,Beijing 100044,China;National Demonstration Center for Experimental Water Environment Education(Beijing University of Civil Engineering and Architecture),Beijing 100044,China;Beijing Service Bureau for Diplomatic Missions(BDS),Beijing 100010,China;College of Mathematics&Statistics,Hebei University of Economics and Business,Shijiazhuang 050061,China;Beijing General Municipal Engineering Design&Research Institute Co.,Ltd.,Beijing 100082,China)

机构地区：[1]北京建筑大学北京未来城市设计高精尖创新中心,北京100044 [2]北京建筑大学水环境国家级实验教学示范中心,北京100044 [3]北京外交人员服务局,北京100010 [4]河北经贸大学数学与统计学学院,河北石家庄050061 [5]北京市市政工程设计研究总院有限公司,北京100082

出　　处：《应用化工》2020年第4期797-800,813,共5页Applied Chemical Industry

基　　金：国家自然科学基金(51678026);北京建筑大学研究生创新项目资助(PG2019044)。

摘　　要：以微生物指标中代表性细菌大肠杆菌作为研究对象,探究EGCG及其络合物EGCG-Cu的抑菌活性及其影响因素。结果表明,EGCG-Cu与EGCG的最小抑菌浓度(MIC)分别为200 mg/L和1000 mg/L,EGCG-Cu络合物的抑菌能力优于EGCG。分析初始浓度和反应时间对EGCG-Cu和EGCG抑菌效果的影响表明,与EGCG相比,EGCG-Cu在较低浓度的抑菌效果更明显,但EGCG和EGCG-Cu达到较稳定抑菌率的反应时间均是6 h。通过测定大肠杆菌胞内活性氧簇(ROS)得出,EGCG-Cu诱导的氧化应激效应是其具有优异杀菌性的主要原因。综上表明,EGCG与金属离子络合可能是改进EGCG消毒效果的有效途径。Taking E.coli as the research object,which is the most representative bacteria among the microbiological indicators.The antibacterial activity of EGCG and EGCG-Cu and their influencing factors were explored.The minimum inhibitory concentration(MIC)of EGCG-Cu and EGCG were 200~1000 mg/L respectively,and the bacteriostatic ability of the EGCG-Cu compleXwas found to be better than EGCG.The antibacterial effect of initial concentration and reaction time on EGCG-Cu and EGCG were analyzed.It was found that compared with EGCG,EGCG-Cu had more obvious antibacterial effect at lower concentration.However,the reaction time for EGCG and EGCG-Cu to achieve a stable bacteriostatic rate was 6 h.The reason for the excellent bactericidal ability of EGCG-Cu compleXwas investigated by measuring reactive oxygen species(ROS)in E.coli.The results indicated that EGCG-Cu induced oxidative stress,which was the main reason for its excellent bactericidal properties.In summary,the complexation of EGCG with metal ions may be an effective way to improve the disinfection of EGCG.

关 键 词：EGCG EGCG-Cu 络合 消毒效果 

分 类 号：TQ42[化学工程] TQ2