外周血miR-26a-5p水平变化与高血压性左心室肥厚的关系分析  

Relationship Between Changes of Peripheral Blood miR-26a-5p Levels and Hypertensive Left Ventricular Hypertrophy

作　　者：刘晓霞 吴宝全 LIU Xiaoxia;WU Baoquan(Department of Geriatrics,The First Hospital of Zhangjiakou City,Zhangjiakou 075000,Hebei,China;Cardiology Department,The First Hospital of Zhangjiakou City,Zhangjiakou 075000,Hebei,China)

机构地区：[1]张家口市第一医院老年病科,河北张家口075000 [2]张家口市第一医院心内科,河北张家口075000

出　　处：《心血管病学进展》2020年第5期556-562,共7页Advances in Cardiovascular Diseases

基　　金：河北省科技厅卫生项目(1821094D)。

摘　　要：目的分析外周血miR-26a-5p水平与高血压性左心室肥厚的关系。方法招募符合条件的高血压患者98例,其中单纯高血压患者66例(高血压组),高血压伴左心室肥厚患者32例(左心室肥厚组),并选正常健康志愿者30例作为对照组,比较各组收缩压和舒张压差别;并采集空腹静脉血,实时荧光定量逆转录聚合酶联反应法测定外周血miR-26a-5p水平,按左心室肥厚程度分组,比较不同左心室肥厚程度患者外周血miR-26a-5p水平的差异;超声心动图测定舒张期左心室后壁厚度(LVPWTd)、室间隔舒张末期厚度(LVSD)和舒张末期左心室内径(LVEDD),计算左心室质量(LVM)及左心室质量指数(LVMI),比较高血压组与左心室肥厚组以上各参数的差异,单因素和多因素分析筛选高血压患者发生左心室肥厚的危险因素,受试者工作特征曲线评估miR-26a-5p诊断高血压性左心室肥厚的效能,Pearson相关分析法分析高血压性左心室肥厚miR-26a-5p与超声心动图参数的关系。结果外周血miR-26a-5p比较:左心室肥厚组(0.365±0.097)和高血压组(0.725±0.163)外周血miR-26a-5p低于对照组(0.973±0.105),左心室肥厚组又低于高血压组,P<0.05,高血压伴重度左心室肥厚患者外周血miR-26a-5p(0.276±0.065)水平>中度组(0.357±0.095)>轻度组(0.463±0.112),P<0.05;收缩压比较:左心室肥厚组[(171.15±27.96)mm Hg]>高血压组[(142.63±16.52)mm Hg]>对照组[(115.23±7.43)mm Hg],P<0.05;超声心动图参数比较:左心室肥厚组LVPWTd、LVSD、LVEDD、LVMI[(11.05±0.89)mm、(11.76±1.23)mm、(48.63±3.79)mm、(53.67±5.98)g/m 2.7]高于高血压组[(9.95±1.02)mm、(10.46±1.63)mm、(44.23±4.07)mm、(37.71±6.52)g/m 2.7]与对照组[(9.76±1.21)mm、(10.32±1.74)mm、(43.78±5.17)mm、(36.51±5.75)g/m 2.7],P<0.05;年龄(OR=1.420)、高血压病程(OR=1.706)、血尿酸(OR=1.306)及miR-26a-5p(OR=1.502)均为高血压患者出现左心室肥厚的相关影响因素,P<0.05;诊断效能:miR-26a-5p cut-off值≤0.4Objective To analyze the relationship between peripheral blood miR-26a-5p level and hypertensive left ventricular hypertrophy.Methods 98 eligible patients with hypertension were recruited,including 66 with simple hypertension(hypertension group)and 32 with hypertensive left ventricular hypertrophy(left ventricular hypertrophy group),and 30 normal healthy volunteers were selected as the control group.The systolic blood pressure and diastolic blood pressure were compared among groups.Fasting venous blood of all subjects was collected to determine the peripheral blood miR-26a-5p level by real-time fluorescent quantitative reverse transcription polymerase chain reaction.According to the degree of left ventricular hypertrophy,the patients were grouped,and peripheral blood miR-26a-5p levels in patients with different degrees of left ventricular hypertrophy were compared.The left ventricular end diastolic posterior wall thickness(LVPWTd),ventricular septal end diastolic thickness(LVSD)and left ventricular end diastolic diameter(LVEDD)were measured with echocardiography.The left ventricular mass(LVM)and left ventricular mass index(LVMI)were calculated.The above parameters in hypertension group and left ventricular hypertrophy group were compared.Risk factors for left ventricular hypertrophy in patients with hypertension were screened through univariate and multivariate analyses.The efficiency of miR-26a-5p in diagnosing hypertensive left ventricular hypertrophy was evaluated with the receiver operating characteristic curve.Pearson correlation analysis was conducted to analyze the relationship between hypertensive left ventricular hypertrophy miR-26a-5p and echocardiographic parameters.Results The order of peripheral blood miR-26a-5p from low to high was as follows:left ventricular hypertrophy group(0.365±0.097),hypertension group(0.725±0.163),control group(0.973±0.105)(P<0.05).The order of peripheral blood miR-26a-5p level in patients with hypertension and different degrees of left ventricular hypertrophy from high to

关 键 词：高血压 左心室肥厚 微小RNA-26a-5p 诊断 血管重塑 

分 类 号：R544.1[医药卫生—心血管疾病]