15种免疫增效剂对鸡新城疫免疫效果影响的对比研究  

Comparative study of effect on immune efficiency to Newcastle disease with fifteen intensifiers

作　　者：田澍瑶 杨雨 胡玥 武志伟 张子卉 袁艳阳 李富桂 TIAN Shu-yao;YANG Yu;HU Yue;WU Zhi-wei;ZHANG Zi-hui;YUAN Yan-yang;LI Fu-gui(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津300384

出　　处：《天津农学院学报》2020年第1期50-52,57,共4页Journal of Tianjin Agricultural University

基　　金：国家级大学生创新训练计划项目(201810061116)。

摘　　要：采用新城疫(ND)HI抗体效价作为指示来研究15种免疫增效剂的功效。试验首先对160只试验鸡进行采血,用HI方法测定ND抗体效价,然后接种注射ND-IB-EDS灭活油乳剂三联疫苗,并将试验鸡随机分成16组,试验1-15组别给予不同的免疫增效剂,而对照组则口服等量的生理盐水;在给药后第0、2、4、6、8、10、12、14、17、22、28天分别采血,采用微量HI试验检测ND抗体效价,并用SPSS软件进行差异显著性分析。统计学分析后比较其对免疫效果的影响。结果表明:15种免疫增效剂在早期(用药后第4-12天)均对鸡ND的免疫效果有增强作用,抗体效价均高出对照组1~2个HI抗体滴度。有2种免疫增效剂在给药后第四天、6种在给药后第八天时与对照组相比差异均显著(0.01<P<0.05)。综合考虑,在养禽业生产实践中建议首选鸡转移因子。Immune efficiency of 15 different immunopotentiators were contrastively studied in this paper with Newcastle disease(ND)antibody titre as indicator.160 pullets were divided randomly into 16 groups with ND-EDS-IB attenuated oil-emulsion vaccine injected,and 15 experimental groups were respectively administrated with different immune intensifiers while the control group was given equivalent saline.Blood samples were collected on 0th,2nd,4th,6th,8th,10th,12th,14th,17th,22nd,28th day for ND HI antibody titres detection,and the effects on immunity were compared after statistical analysis by software SPSS.The results showed that 15 immunopotentiators appeared obviously immuno-efficiacy in early period(4-12 days post-administration)with 1-2 HI titres higher than control’s;There are significant differences(0.01<P<0.05)in 2 kinds of immunopotentiators on 4th day and 6 kinds on 8th day.Chicken transfer factor will be firstly recommended to apply in poultry industry following comprehensive analysis.

关 键 词：免疫增效剂 新城疫抗体效价 脂多糖 鸡转移因子 四合一干扰素 灵芝孢子粉 

分 类 号：S852.4[农业科学—基础兽医学]