不同溶血性大肠杆菌的分离与鉴定  

Isolation and identification of two hemolytic strains of canine Escherichia coli

作　　者：杜荣起 任君 乔启 张晴 刘燕霏 杨建德 DU Rong-qi;Ren Jun;QIAO Qi;ZHANG Qing;LIU Yan-fei;YANG Jian-de(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津300384

出　　处：《天津农学院学报》2020年第1期61-64,75,共5页Journal of Tianjin Agricultural University

基　　金：天津农学院大学生创新创业项目(201810061116);天津自然科学基金项目(07JCYBJC16000)。

摘　　要：为明确伴侣动物化脓性疾病的致病原因,对某动物医院门诊两只母犬的子宫脓性分泌物进行病原微生物学鉴定。通过观察病料接种培养基菌落形态、革兰氏染色镜检、生化试验、药敏试验以及16S rRNA基因序列比对分析,分离菌分别为α溶血和β溶血性大肠埃希氏菌。β溶血性大肠杆菌对卡那霉素、链霉素、头孢噻吩等耐药,α溶血性大肠杆菌仅对麦迪霉素耐药,β溶血菌株耐药性明显高于α溶血菌株,为防治溶血性大肠杆菌引起的相关感染提供参考依据。In order to clarify the pathogenic causes of purulent diseases of companion animals,the pathogenic microbiological identification of uterine purulent secretions of two female dogs in an outpatient department of an animal hospital was carried out.Colony morphology,gram staining microscopy,biochemical test,drug susceptibility test and 16S rRNA gene sequence alignment analysis of the inoculated medium were observed.The isolates were alpha hemolytic and beta hemolytic Escherichia coli,respectively.Beta hemolytic Escherichia coli was resistant to kanamycin,streptomycin and cefothiophene.Alpha hemolytic Escherichia coli was only resistant to medilamycin.The resistance of beta hemolytic strain is significantly higher than that of alpha hemolytic strain,which provides a reference for the prevention and treatment of related infections caused by hemolytic Escherichia coli.

关 键 词：溶血性大肠杆菌 16S rRNA 分离鉴定 溶血素 

分 类 号：S858.611[农业科学—临床兽医学]