光照、氮素和高盐胁迫对雨生红球藻积累虾青素的影响研究  

Research on Effects of Light,Nitrogen and High Salt Stress on Accumulation of Astaxanthin in Haematococcus pluvialis

作　　者：陈家宇 李嘉仪 窦勇 吴琳 任虹烨 闫永芳 周文礼[1] 于士国 CHEN Jia-yu;LI Jia-yi;DOU Yong;WU Lin;REN Hong-ye;YAN Yong-fang;ZHOU Wen-li;YU Shi-guo(Tianjin Key Lab of Aquaculture Ecology and Cultivation,Tianjin Agriculture University,Tianjin 300384,China;Tianjin Yunhua Agricultural Technology Development Co.,Ltd.,Tianjin 301823,China)

机构地区：[1]天津农学院天津市水产生态及养殖重点实验室,天津300384 [2]天津蕴华农业科技发展有限公司,天津301823

出　　处：《食品研究与开发》2020年第12期75-83,共9页Food Research and Development

基　　金：天津市大学生创新创业训练计划项目(201810061091);天津市水产产业技术体系创新团队项目(ITTFRS2017005);天津市优秀科技特派员支持项目(18ZXBFNC00170);天津市水产生态及养殖重点实验室开放基金项目(TJAE201805);天津市宝坻区农业科技计划重大项目(201810)。

摘　　要：为摸索雨生红球藻积累虾青素的最优条件,研究光照强度、NaNO3和NaCl浓度对雨生红球藻光合作用和积累虾青素的影响,结果表明:不同光照强度处理组最大光能转化效率(Fv/Fm)较对照组均显著降低(P<0.05);试验第24天,光照强度为236μmol/m^2/s的试验组微藻积累虾青素达最高值9.01 mg/L;NaCl浓度为10 g/L和12.5 g/L时雨生红球藻Fv/Fm和有效光能转化效率值(Yield)均低于对照组,而NaCl浓度为5 g/L时微藻Fv/Fm和Yield值均高于对照组,NaCl浓度为5、7.5、10 g/L时微藻积累虾青素水平显著高于其他试验组(P<0.05),试验第24天,NaCl浓度为7.5 g/L时,微藻积累虾青素含量达到最高,较对照组提高了34.4%;NaNO3浓度为0、0.032、0.065 g/L时,雨生红球藻Fv/Fm整体呈先上升后缓慢降低的趋势,较对照组差异显著(P<0.05),在氮胁迫处理条件下雨生红球藻积累虾青素含量逐渐升高,试验第22天后细胞内虾青素水平渐趋稳定,当NaNO3浓度为0时细胞内虾青素含量显著高于其他试验组(P<0.05)。In order to explore the optimal conditions for the accumulation of astaxanthin by Haematococcus pluvialis,the effects of light intensity,NaNO3 and Na Cl concentration on photosynthesis and the accumulation of astaxanthin were studied and the results showed that Fv/Fm in the treatment group with different light intensity was significantly lower than that in the control group(P<0.05);on the 24 th day of the experiment,the maximum value of the astaxanthin accumulated by microalgae in the experimental group with the light intensity of 236μmol/m^2/s was 9.01 mg/L.Fv/Fm and Yield value of H.pluvialis at Na Cl concentrations of 10,12.5 g/L were lower than those of the control group,while Fv/Fm and Yield values of H.pluvialis at Na Cl concentrations of 5 g/L were higher than those of the control group.When Na Cl concentration was 5,7.5,10 g/L,the content of astaxanthin accumulated in microalgae was significantly higher than that in other experimental groups(P<0.05);on the 24 th day of the experiment,when Na Cl concentration was 7.5 g/L,the content of astaxanthin accumulated in microalgae reached the highest level,34.4%higher than that in the control group.When the concentration of NaNO3 was 0,0.032,0.065 g/L,the Fv/Fm of H.pluvialis presented an overall trend of rising first and then slowly decreasing,which was significantly different from that of the control group(P<0.05).Under nitrogen stress condition,the content of astaxanthin accumulated by H.pluvialis increased gradually,and the astaxanthin level within cells became stable after 22 days.When NaNO3 concentration was 0,the astaxanthin content within cells was significantly higher than that of other experimental group(P<0.05).

关 键 词：雨生红球藻 光胁迫 氮胁迫 盐胁迫 荧光参数 

分 类 号：Q945[生物学—植物学]