海狸鼠源性成分的PCR鉴别研究  

Identification Study of Myocastor coypus Components by PCR Method

作　　者：段庆梓 张玉 王巍 尚柯 梁恒兴 DUAN Qing-zi;ZHANG Yu;WANG Wei;SHANG Ke;LIANG Heng-xing(Chengdu Institutes for Food and Drug Control,Chengdu 610045,Sichuan,China)

机构地区：[1]成都市食品药品检验研究院,四川成都610045

出　　处：《食品研究与开发》2020年第12期181-184,共4页Food Research and Development

基　　金：成都市科技惠民应用示范项目(2015-HM02-00099-SF)。

摘　　要：以海狸鼠和其他物种的细胞色素b基因为目标基因,通过序列比对在碱基差异明显的区域设计海狸鼠的特异性引物,并通过退火温度、反应循环数、方法检出限和特异性等参数的考察建立海狸鼠源性成分的聚合酶链式反应(polymerase chain reaction,PCR)鉴别方法。结果显示:该PCR方法对海狸鼠能够扩增出约599 bp的条带,对其他物种无任何扩增现象,且在鸡肉和兔肉中对海狸鼠成分的检出限可达到0.1%。这表明该方法有较好的特异性和较高的灵敏度,能够满足海狸鼠源性成分的检测要求。The specific primer was designed by cytochromeb gene alignmnet between Myocastor coypus and other species,which was used to establish the polymerase chain reaction(PCR)detection of Myocastor coypus by different conditions and parameters of annealing temperature,reaction cycles,detection limit and specificity of the method.The PCR results showed that the method could amplify about 599 bp bands of Myocastor coypus,without any amplification of other species,and the detection limit of Myocastor coypus components could reach to 0.1%in chicken and rabbit.This PCR method had good specificity and sensitivity,which could meet the detection requirements of Myocastor coypus components.

关 键 词：海狸鼠 源性成分 聚合酶链式反应(PCR)鉴别 CYTB基因 检出限 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]