急性酒精性肝损伤模型的考察及验证  

Investigation and verification of acute alcoholic liver injury model

作　　者：杨柳[1] 王文地 马允 王文全[1,2,3] 侯俊玲[1,3] 崔洁[2] YANG Liu;WANG Wendi;MA Yun;WANG Wenquan;HOU Junling;CUI Jie(School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 102488,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;Engineering Research Center of Standardized Production for Chinese Crude Drugs,Ministry of Education,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]中药材规范化生产教育部工程研究中心,北京102488

出　　处：《西北药学杂志》2020年第3期391-396,共6页Northwest Pharmaceutical Journal

基　　金：国家重点研发计划资助项目(编号:2018YFC1706500)。

摘　　要：目的考察急性酒精性肝损伤模型的稳定性,便于后续科学有效地进行相关新药的开发研究。方法比较2种急性酒精性肝损伤模型,并对一次性灌胃体积分数为50%的乙醇制备的酒精性肝损伤模型进行稳定性研究,通过联苯双酯滴丸进行验证。结果2种急性酒精性肝损伤模型肝损伤程度比较差异无统计学意义(P>0.05),随着取材时间的延长,肝损伤程度有所减轻,联苯双酯滴丸在一次性灌胃体积分数为50%的乙醇制备的酒精性肝损伤模型中对酒精性肝损伤具有保护作用。结论一次性灌胃体积分数为50%的乙醇可建立急性酒精性肝损伤模型。Objective The article is to investigate the stability of acute alcoholic liver injury model,which is convenient for follow-up scientific and effective research and development of related new drugs.Methods 2 models of acute alcoholic liver injury were compared.The stability of alcoholic liver injury model prepared by 50%ethanol in one-time gavage was studied and verified by Bifendate Pills.Results There was no significant difference in the degree of liver injury between the 2 models of acute alcoholic liver injury(P>0.05).The degree of liver injury decreased with the extension of the time taken.Bifendate Pills showed protective effects on alcoholic liver injury under the model of alcoholic liver injury prepared by 50%ethanol in one-time.Conclusion The results show that a single oral gavage of 50%ethanol can more easily establish a model of acute alcoholic liver injury.

关 键 词：联苯双酯滴丸 急性酒精性肝损伤 动物模型 

分 类 号：R965[医药卫生—药理学]