超声波辅助/疏水性低共熔溶剂萃取-高效液相色谱法测定牛奶中的多环芳烃  

Determination of Polycyclic Aromatic Hydrocarbons in Milk by Ultrasonic-assisted Hydrophobic Deep Eutectic Solvent Extraction Combined with High Performance Liquid Chromatography

作　　者：何婷婷 周彤[1] 谭婷 万益群 HE Tingting;ZHOU Tong;TAN Ting;WAN Yiqun(Jiangxi Province Key Laboratory of Preventive Medicine,School of Public Health,Nanchang University,Nanchang 330006;Center of Aanlysis and Testing,Nanchang University,Nanchang 330047)

机构地区：[1]江西省预防医学重点实验室,南昌大学公共卫生学院,江西南昌330006 [2]南昌大学分析测试中心,江西南昌330047

出　　处：《分析科学学报》2020年第2期177-182,共6页Journal of Analytical Science

基　　金：国家重点研发计划专项(No.2018YFC1604306);国家自然科学基金(No.31660483)。

摘　　要：本文以四丁基氯化铵和正辛醇制备的疏水性低共熔溶剂为绿色提取溶剂,结合高效液相色谱-荧光检测技术,构建了同时分析牛奶中芴、荧蒽、蒽、芘、[艹屈]和苯并[a]芘6种多环芳烃类物质的新方法。系统地考察了各种萃取条件,包括萃取剂的种类、萃取时间、萃取温度、萃取剂用量以及盐的含量对牛奶中6种多环芳烃化合物提取效果的影响。结果表明,在优化的实验条件下,采用所制备的低共熔溶剂体系能较好地萃取上述6种多环芳烃化合物,三个水平的加标回收率为70.87%~100.45%,相对标准偏差为1.05%~8.95%,方法的检出限在0.03~0.3μg/kg之间。该方法快速、简便、环保,适用于牛奶中多环芳烃的测定。In the study,a new method was developed for the determination of polycyclic aromatic hydrocarbons(PAHs)in milk samples by hydrophobic deep eutectic solvent extraction combined with high performance liquid chromatography-fluorescence detector(HPLC-FLD).The extraction conditions such as extraction time,temperature,volume and amount of salt were optimized systematically.Under the optimal conditions,the limits of detection were in the range of 0.03-0.3μg/kg,with the relative standard deviations(RSDs)of 1.05%-8.95%.The developed method is rapid,simple and environmental friendly and suitable for determination of polycyclic aromatic hydrocarbons in milk.

关 键 词：疏水性低共熔溶剂 高效液相色谱-荧光检测 牛奶 多环芳烃 

分 类 号：O657.72[理学—分析化学]