氯霉素磁性分子印迹聚合物的合成及应用  

Preparation and Application of Chloramphenicol Magnetic Molecular Imprinted Polymers

作　　者：章琦 赵薇[1] 韩迎红 ZHANG Qi;ZHAO Wei;HAN Yinghong(College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072)

机构地区：[1]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《分析科学学报》2020年第2期212-216,共5页Journal of Analytical Science

摘　　要：本文以氯霉素(CAP)为模板分子,磁性Fe3O4纳米颗粒为支撑载体,用表面沉淀聚合法制备磁性分子印迹聚合物(Magnetic Molecularly Imprinted Polymers,MMIPs)。利用透射电镜(TEM)、傅里叶红外(FT-IR)光谱对聚合物表征。在磁铁颗粒表面合成厚度约为100 nm的分子印迹层,实际应用中聚合物可以实现磁分离收集。通过一系列吸附实验探究聚合物的吸附性能,证明MMIPs比其磁性非印迹聚合物(Magnetic Non-molecularly Imprinted Polymers,MNIPs)富集目标分子的能力更强,达到吸附平衡的时间仅为MNIPs所需时间的六分之一。将该聚合物应用于牛奶中氯霉素含量的检测,加标回收率为97.3%~104.3%,相对标准偏差(RSD)在2.7%~4.2%之间,检出限(LOD)为2.7μg/kg,定量限(LOQ)为8.9μg/kg。该分子印迹聚合物可用于牛奶中氯霉素前处理富集,操作简单快捷,灵敏度高。A magnetic molecular imprinted polymers(MMIPs)was synthesized by using the method of surface precipitation polymerization with chloramphenicol(CAP)as template molecule.The prepared MMIPs was characterized by transmission electron microscopy,Fourier transform infrared spectroscopy and vibrating sample magnetometer.The results indicated that a uniform and stable MMIPs was grafted onto the surface of Fe3O4 magnetic nanoparticles with a thickness of about 100 nm.The synthesized MMIPs possessed good magnetism,which could be easily separated by applying an external magnetic field.Experiments were also shown that MMIPs was more capable of enriching target molecules than their non-imprinted polymers(MNIPs),and the time to reach adsorption equilibrium was one-sixth of the time required for MNIPs,and the maximum adsorption capacity was up to 30.5 mg/g.With the prepared MMIPs as adsorbent,a method of magnetic solid phase extraction-high performance liquid chromatography was developed.The limit of detection calculated on the basis of the CAP concentration producing a signal/noise ratio of 3∶1 was 2.7μg/kg,and the limit of quantitation producing a signal/noise ratio of 10∶1 was 8.9μg/kg.The developed method is simple,quick,and sensitive and can be used for determination chloramphenicol in milk samples.

关 键 词：氯霉素 分子印迹 磁分离 吸附 

分 类 号：O657.72[理学—分析化学]