用氮掺杂碳点为荧光探针检测对羟基苯甲醛  

Nitrogen-doped carbon dots used as fluorescent probe to detect p-hydroxy benzaldehyde

作　　者：弓辉[1] 张梦婷 刘丹 杨学芳 卞伟[1] GONG Hui;ZHANG Meng-ting;LIU Dan;YANG Xue-fang;BIAN Wei(Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学基础医学院,山西太原030001

出　　处：《化学研究与应用》2020年第5期717-724,共8页Chemical Research and Application

基　　金：山西医科大学博士启动基金项目(03201514)资助;教育教学改革研究项目(XJ2018004)资助;山西省自然科学基金项目(201901D111210)资助。

摘　　要：本文通过一步水热法,以菊花为原料,制备了氮掺杂碳点(N-CDs),并对N-CDs的粒径分布、元素组成、形貌结构及表面官能团进行了表征,考察了N-CDs的光谱性质。本实验基于PHBA有效猝灭N-CDs荧光的现象,以N-CDs为荧光探针,对PHBA进行了定量测定。结果显示该探针对PHBA的检测线性范围为10.0-150.0μM,检出限为63 nM。常见的离子、氨基酸和糖类对N-CDs检测PHBA基本无影响。同时,初步探讨了N-CDs与PHBA的反应机理主要为静态猝灭和内滤光效应。将该探针应用于健康人体血样和尿样中PHBA的微量测定。本实验为对羟基苯甲醛的检测提供了新的思路,也预示了该荧光探针在生物样品检测方面具有广阔的应用前景。In this paper,nitrogen-doped carbon dots(N-CDs)were prepared by one-step hydrothermal method using chrysanthemum as original material.The particle size,composition,morphology and functional groups of the N-CDs were characterized.Meanwhile the spectral properties of N-CDs were investigated.Based on the phenomenon of fluorescence quenching of N-CDs by PHBA,PHBA was quantitatively determined using N-CDs as fluorescence probe.It shows that the linear range was 30.0~150.0μM with the limit of detection(LOD)of 63 nM.The mechanism between N-CDs and PHBA was mainly static quenching and internal filtering.Common ions,amino acids and saccharides have no effect on the detection of PHBA by N-CDs.The fluorescence probe was successfully used to detect the trace PHBA in blood samples and urine samples of healthy adults.This experiment provided a new idea for the detection of PHBA and anticipated that the fluorescence probe has a broad application prospect for the detection of biological samples.

关 键 词：氮掺杂碳点 对羟基苯甲醛 荧光探针 荧光猝灭机理 

分 类 号：O657.3[理学—分析化学]